Master of Science I (Botany) Examination: Oct Nov 2016
Semester I (New CBCS)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SM
126
Wednesday
23/11/2016
10:30 AM
to
01:00 P.M
Tools And Techniques
in Botany
C
SCT 1.1
Instructions: Attempt totally five questions.
Question no. 1 is compulsory (section
Attempt any two questions from question no. 2 to 4 (section II)
Attempt any two questions from question no. 5 to 7 (section
III)
Figures to the right indicate full marks.
Total Marks: 70
SECTION 1
Q.1 Rewrite the following sentences by choosing correct alternative. 14
Presentation of biological data is used.
Line diagram Bar diagram
Pie diagram All of these
The lens that is within the eyepiece of the light microscope is called

Scanning High power
Low power Ocular
lamp is used as a source of visible radiation.
Hydrogen Nerst glower
Tungsten Deuterium
To measure the absorption of a compound in UV region.
Glass Quartz
Plastic All of these
The application of statistical methods in biology is called
Statics in Biology Statistics in vivo
Biostatistics Biological statistics
is regarded as father of biostatics.
Fischer Karl person
Francis Galton Francis Bacon
To determine the height of person when his weight is given

Correlation problem Association problem
Regression problem Qualitative problem
The absorption maximum shifts towards higher wavelengths due to the
presence of auxochrome is called
Bathochromic shift Hypsochromic shift
Hyperchromic shift Hypochromic shift
Page 1 of 2
In gel filtration chromatography the particles are separated based on
Affinity Ion
Molecular size Gel size
10) In herbarium technique the specimen preparation involves
Strapping Sewing
Special handling All of these
11) Handling of incoming specimens in herbarium maintenance
Fumigation Heating
Poisoning All of these
12) factors affecting electrophoretic mobility.
The sample Electric field
The buffer All of these
13) is used to measure the radioactivity.
SEM UV spectrophotometer
Scintillation counters NMR
14) Photograph which is taken form microscope is known as
Macrograph Monograph
Micrograph Pictograph
SECTION II
Q.2 What is chromatography? Describe the working principle of HPLC. 07
Discuss the principle and application flame spectrophotometry. 07
Q.3 Write the principle and applications of fluorescene microscope. 07
Explain how permanent slides are prepared. 07
Q.4 Write the principles and applications of Colorimetry. 07
Explain principles and applications of gel filtration. 07
SECTION III
Q.5 Herbarium preparation technique. 05
Applications of gas chromatography. 05
Write a note on cytophotometry. 04
Q.6 Types of presentation of biological data. 05
Analysis of variance. 05
Stains used in cytological techniques. 04
Q.7 Write a notes on any three: 14
Application of atomic absorption.
Important herbaria in India.
Theory of probability.
Half life of radioisotopes.