M.Sc. (Semester (CBCS) Examination Nov/Dec-2018
Botany
TOOLS AND TECHNIQUES IN BOTANY
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labeled diagrams whenever necessary.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
The basic theory of atomic absorption states that
The incident light intensity is directly proportional to atom concentration
Light is absorbed by ground-state electrons
The transmitted light is proportional to the ground state atoms
Absorption results in the lowering of energy levels of most atoms
is the most suitable gas to use as a carrier gas in a gas chromatogram.
Helium Oxygen
Methane Both a and c
HPLC stands for
High Pressure Liquid Chromatography
High Performance Liquid Chromatography
Both a and b
Highly placed Liquid Chromatography
The ultracentrifuge method for determining the molecular weight of proteins
was discovered by
Svedberg Tiselius
Schraiber Bouguer
The biggest herbarium in India is
I.A.R.I. Delhi
Central National Herbarium, Calcutta
St. Xavier's Herbarium, Bombay
Forest Research Institute, Dehradun
Isotopes of an element
May or may not be radioactive
Have the same atomic number but differing atomic masses
May be used for human disease diagnostics
All of the above
A Geiger-Muller counter is able to provide an indirect measure of radioactivity
because radiation has a property of
Ionization Making matter glow in the dark
Fogging photographic film Attracting electrons
The main advantage of fluorescence over UV-Vis spectroscopy is
Its sensitivity
Its compatibility with separation techniques
Its compatibility with most analysts
None of these
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Chi square test is
Measure the degree of deviation of the experimental result from the
expected result
To test the closeness of observed and expected frequency
To test the population variance and sample variance
All of these
10) Living, unstained cells and organism can be observed best using
Fluorescent microscopy TEM
Phase contrast microscopy Scanning electron microscope
11) Which of the following identifies three types of sources used in AAS?
Hollow Cathode Lamp Electrodeless Discharge Lamp
Argon Lamp
Electrodeless Discharge Lamp Deuterium lamp, Hollow
Cathode Lamp
Deuterium lamp, plasma, flame
Neon lamp, Acetylene torch, Tungsten lamp
12) Scanning electron microscopy is best used to study
Small internal cell structures Internal structure of live, motile cells
Surface morphology All of the above
13) In distribution probability of success remains constant from trial to trial.
Normal Poisson
Binomial None of these
14) chemical is used for poisoning the specimens in herbarium technique.
KCl AgNO3
HCI HgCl2
Q.2 Answer the following:- (Any four) 08
Define pH and give its scale.
What is probability?
Give any two application of phase contrast microscopy.
Write any two application of gel electrophoresis.
What is half-life of radio isotopes?
Write notes on. (Any two) 06
Principle o Flame spectrophotometry
O-banding
Effect of radiation on biological system
Q.3 Answer the following:- (Any two) 08
Describe coefficient of variation.
Explain principle of electron microscope.
Write a note on radioactivity counting system.
Answer the following:- (Any two) 06
Write application of computer in life science.
Describe herbarium technique.
Explain important herbaria in India.
Q.4 Answer the following:- (Any two) 10
Describe photomicrography.
Explain Chi-square test.
Explain thermal inactivation point.
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Answer the following:- (Any one) 04
Write application NMR spectroscopy.
Write application ESR spectroscopy.
Q.5 Answer the following:- (Any two) 14
Describe principle and application atomic absorption.
Describe principle and application of ultracentrifugation.
Describe principle and application of SEM.