M.Sc. (Semester (CBCS) Examination Mar/Apr-2018
Botany
TOOLS AND TECHNIQUES IN BOTANY
Time: 2½ Hours
Max. Marks: 70
Instructions: Question 1 is compulsory. Attempt any two questions from Q.2, 3 4 Attempt any two short notes questions from Q.5, 6 7 Draw neat and labeled diagrams wherever necessary. Figures to the right indicate full marks.
Q.1
Rewrite the following sentences by choosing correct alternative:-
14
Biostatistics is also called as
Statistics in biology
Biometry
Statistics in vivo
Bionemerology
Variables whose value can be expressed numerically are called
Quantitative variable
Qualitative variable
Absolute variable
Continuous variable
Correlation coefficient is a number between
and
0 and

and 0
and
light source is used in Fluorescence microscopy.
Xenon are lamps
Mercury-vapor lamps
UV lamps
Both a b
C18 columns are used in chromatography.
HPLC
Gel filtration
Ion Exchange
Affinity
Sodium and potassium metal ions concentration is determined using instrument.
NMR
Flame spectrophotometry
ESR
UV
DNA absorbs light in nm.
100
200
260
280
The absorption maximum shifts towards higher wavelengths due to the presence of auxochrome is called
Bathochromic shift
Hypsochromic shift
Hyperchromic shift
Hypochromic shift
technique uses magnetic properties of atomic nuclei.
Colorimetry
Spectrophotometry
O
NMR
Fluorescence
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SLR-UD-115
10) is used extensively for radiation protection.
Fluorometry
Dosimetry
Nephelometry
Potentiometry
11) Handling of incoming specimens in herbarium maintenance involves
Fumigation
Heating
Poisoning
All of these
12) instrument is used for detecting and measuring radioactivity.
Scintillation counter
Atomic absorption spectroscopy
Flame spectrophotometer
Ultra sonicator
13) ANOVA stands for
Analysis of variables
Analysis of variance
Analysis of variety
Anatomical variance
14) is used to study internal structure of cells is
Scanning electron microscope
Transmission electron microscope
Light microscope
Compound microscope
Q.2
Write about:-
Principle and applications of Immuno fluorescence microscopy.
What is TEM? Describe the working principle of Transmission electron microscope.
14
Q.3
Write the working principle and applications of UV-VIS spectrophotometry.
14
Explain Principle and applications of HPLC.
Q.4
Explain:-
Write the principle and applications of Immuno fluorescence microscopy.
Explain the effect of radiation on biological systems.
14
Q.5
Write a short note
14
Applications of computer in life sciences
Herbarium Preparation technique
Uses of phase contrast microscope
Q.6
Write a short note
14
Types of presentation of biological data
Ultracentrifugation
Buffers and its uses
Q.7
Write a short note
14
Radioisotopes
Important herbaria in India
Binomial distributions