M.Sc. (Semester (CBCS) Examination Oct/Nov-2017
Botany
TOOLS AND TECHNIQUES IN BOTANY
Day Date: Thursday, 23-11-2017 Max Marks: 70
Time: 10.30 AM to 01.00 PM
Instructions: Attempt totally five questions.
Question no.1 is compulsory (Section-I)
Attempt any two questions from question no.2 to 4(Section-II)
Attempt any two questions from question no.5 to 7(Section-III)
Figures to the right indicate full marks.
Q.1 Rewrite the following sentences by choosing correct alternative:- 14
Regression analysis, the variable that is being predicted is the
Dependent variable Independent variable
Intervening variable Is usually x.
Analysis of Variance (ANOVA) is a statistical method used to test
differences between two or more.
Mean Mode
Median Standard Deviation
To determine the height of a person when his weight is given is
Correlation problem Association problem
Regression problem Qualitative problem
To study viruses microscope is used.
SEM Inverted
Fluorescence Binocular
Kind of electron microscope which is used to study internal structure of cells
is
Scanning electron microscope
Transmission electron microscope
Light microscope
Compound microscope
Photograph which is taken from microscope is known as
Macrograph Monograph
Micrograph Pictograph
Molecular structure is determined by
Atomic absorption Flame spectrophotometry
ESR spectroscopy NMR
have lowest shorter wavelength.
X-rays UV rays
IR-rays
The absorption maximum shift towards higher wavelengths due to the
presence of auxochrome is called
Bathochromic shift Hypsochromic shift
Hyperchromic shift Hypochromic shift
Page 2 of 2
SLR-MD-126
10) The largest herbarium of the world is located in
New York Kew
Geneva Berlin
11) In herbarium technique the specimen preparation involves
steps.
Strapping Sewing
Special handling All of these
12) Handling of incoming specimens in herbarium maintenance involves
steps.
Fumigation Heating
Poisoning All of these
13) factors affecting electrophoretic mobility.
The sample Electric field
The buffer All of these
14) is used to measure the radioactivity.
SEM UV spectrophotometer
Scintillation counter NMR
SECTION II
Q.2 Explain the theory and application of probability. 07
Write in brief principle and application of transmission electron microscopes. 07
Q.3 What is chromatography? Describe the working principle of HPLC. 07
Discuss the principle and application flame spectrophotometry. 07
Q.4 Write the principles and applications of Immuno fluorescence microscopy. 07
Explain the effect of radiation on biological systems. 07
SECTION III
Q.5 Give account of acidic and basic buffers. 05
Write a note on herbarium maintenance. 05
Write a note on radioisotopes. 04
Q.6 Applications of computer in life sciences. 05
Write a note on herbarium maintenance. 05
Uses of phase contrast microscope. 04
Q.7 Writes note on any three: 14
Principles isoelectric focusing.
Correlation and regression.
Radioactive decay.
Significance of herbarium.