M.Sc. Botany (Semester- (CBCS) Examination, 2017
TOOLS TECHNIQUES IN BOTANY
Day Date: Tuesday, 25-04-2017 Marks: 70
Time: 10.30 AM to 1.00 PM
Instruction Attempt totally five questions.
Question no.1 is compulsory (Section-I)
Attempt any two questions from question no.2 to
4 (Section II).
Attempt any two questions from question No.5
to Section III)
Figures to the right indicate full marks.
SECTION I
Q.1 Rewrite the following sentences by choosing correct
Alternatives.
14
The Application of statistical methods in Biology is called

Statistic in Biology Statistic in vivo
Biostatistics Biological statistics
is regarded as father of Biostatistics.
Fisher Karl Pearson
Francis Galton Francis Bacon
The Correlation coefficient is used to determine
A specific value of the y-variable given a specific value of
The x-variable.
A specific value of the x-variable given a specific value of
The variable.
The strength of the relationship between the x and y
Variables.
No relationship between the x and y variables.
light is suitable for getting maximum resolution.
Red Blue Green Orange
The resolving power of unaided human eye is
1 cm 100 200nm 400nm
of the following is used to visualize live cells.
SEM TEM
Phase contrast microscope All of these
DNA absorbs light in
100 200 260 280
Page 1 of 3
In UV-VIS spectrophotometer resolves
polychromatic radiation into its individual wavelength.
Detector Monochromator
Light source Sample holder
lamp is used as a source of UV rays in
Spectrophotometer.
Hydrogen Deuterium halogen Both a b
10) chromatography is used to separate the molecules
based on charge.
Gel filtration Ion exchange
Affinity Gas
11) In gel filtration chromatography the particles are separated
based on
Affinity Ion Molecular size Gel size
12) technique is used to separate molecules based
on molecular size and charge.
Ultracentrifugation Gas chromatography
HPLC Isoelectric focusing
13) technique is used to study the metabolic pathway in
Living organism.
Tracer NMR
Cytophotometry photomicrography
14) ANOVA stands for
Analysis of variables. Analysis of variance
Analysis of variety Anatomical variance
SECTION II
07
Q,2 Give an account Coefficient of variation. 07
Write in brief principle and applications of fluorescence
microscopy.
Q.3 What is SEM? Describe the working principle of scanning
electron microscope.
07
Discuss the technique of isoelectric focusing 07
Q.4 Write the principles and applications of NMR. 07
Explain how permanent slides are prepared. 07
Page 2 of 3
SECTION III
Q.5 Herbarium preparation technique. 05
Application of radioisotopes in Biology. 05
Write a note on ultracentrifugation 04
Q.6 Types of presentation of biological data 05
Analysis of variance 05
Use of Cystophotometry 04
Q.7 Write notes on any three: 14
Principles UV spectrophotometer.
Application of affinity chromatography
Binomial distribution
Uses of Dosimetry