Master of Science II (Botany) Examination: November 2016
Semester IV (New CGPA)
SLR No. Day
Date Time Subject Name Paper
No. Seat No.
SLR SM
151
Saturday
19/11/2016
02.30 PM
to
05.00 PM
Plant Tissue Culture
Green House Technology
Hydroponics
C
XIV
Instructions: Question no. 1 is compulsory.
Attempt any two questions from question no.2 to 4
Attempt any two questions from question no.5 to 7
Attempt totally five questions.
Figures to the right indicate full marks.
Draw neat and labeled diagram wherever necessary.
Total Marks: 70
Q.1 Rewrite the following sentences by selecting correct answers from given
alternative.
14
The embryo rescue is
treatment of embryo for the elimination of diseases
removing of embryo under controlled condition and formation of new
plant
prevention of onset of dormancy of young embryo
for breaking of dormancy
In tissue culture the needle and forceps are sterilizes through
Water bath at 2000c dry air oven
autoclave sprit lamp
The best method to obtain virus free plant through tissue culture is
Embryo rescue Protoplast culture
Meristem culture anther culture
best frequently used to induce somaclonal variation.
PDB 2,4 D
NAA PEG
an initial for cybrid production.
emasculation fertilization
pollination protoplast isolation and fusion
In the process of protoplast isolation is used to maintain proper
osmotic pressure in the isolation mixture.
sucrose Nacl
Mannitol Phosphate buffer
What is agar?
A nutrient Vitamins
Broth A solidifying agent
Green house plants are photo synthetically more efficient because the high
of
N2 O2
CO2 CO
Page 1 of 2
Application of embryo culture is
Clonal propagation Production of alkaloids
Overcoming hybridization Haploid production
10) Which one of the following cytokinin?
Indole-3-acetic acid Thidiazuron
Dicamba Brassinosteroid
11) The relative humidity in greenhouse for plant growth is
30-50 50-60
20-30 60-80
12) siliceous mineral of volcanic origin used as soil less culture
for green house.
Rock wool Perlite
Peat Glass wool
13) Callus culture formation is promoted by
Excess of NAA Absences of salts
Darkness and sub culturing Proper light and sub culturing
14) The technique of protoplast fusion was given by
Steward Visit and Haberlandt
Carison et al Barski et al
Q.2 Describe the design of commercial plant tissue culture laboratory. 07
Describe operation and management in tissue culture laboratory. 07
Q.3 What is synthetic seed? Describe in details synthetic seed production. 07
Describe application of synthetic seed. 07
Q.4 What are the basic principles of in-vitro culture? 07
Write critically on factor influencing morphogenesis. 07
Q.5 Answer in brief:
Write note on Anther culture 05
Bioreactors 05
Types of green houses 04
Q.6 Write brief notes on:
Fertigationin Greenhouse 05
Cell suspension culture 05
Types of hydroponics 04
Q.7 Write short notes on any three of the following: 14
Embryo culture
Application of Hydroponics
Embryo rescuing
Pest and disease control in greenhouse