M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Botany
PLANT TISSUE CULTURE AND GREEN HOUSE TECHNOLOGY AND
HYDROPONICS
Time: 2½ Hours Max. Marks: 70
Instructions: Question no. 1 is compulsory.
Figures to the right indicate full marks.
Attempt any two questions from Q.2, 3 and 4.
Attempt any two questions from Q.5, 6 and 7.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
application of callus culture.
Cell organ differentiation Somaclonal variation
Isolation of single cell Both a b
The process in which there is addition of fresh nutrient medium draining out
used medium called as
Suspension culture Batch culture
Continuous culture Cell culture
Synchronization of suspension vulture takes place with the help of
Chemical inhibition Colchicines
Starvation All of these
Production of variant clones with new characters this phenomenon is known
as
Totipotency Clonal propagation
Somatic variation Somaclonal variation
Use of immature embryos for in vitro production of hybrids called as
Cybrids Hybrids
Embryo culture Embryo resue
hormone promotes production of secondary metabolite in tissue
culture.
Auxin Cytokinine
GA ABA
media generally preferred for root culture.
White's MS
B5 Nitsch's
enzymes are commercially used enzymes for protoplast isolation.
Cellulase Hemicelluloses
Pectinases All of these
element in MS media required for chlorophyll biosynthesis.
Nitrogen Molybdenum
Zinc Sulphur
10) For callus initiation ratio of auxin to cytokinine is
High Low
Moderate Very low
Page 2 of 2
SLR-VD-108
11) fucogen used for fusion of protoplast.
NaNo3 NaHco3
PEG Both a c
12) application of cybrid.
Environmental tolerance Production of quality
Cytoplasmic male sterility All of these
13) media used for another culture.
White's N6
B5 All
14) Gynogenic haploids were first developed by
Nitsch White
San Noem Thomas
Q.2 Write a note
Meristem culture
Hybridization
07
07
Q.3 Describe in detail:-
Applications of tissue culture
Applications of synthetic seeds
07
07
Q.4 Describe in detail:-
Somatic embryogenesis
Factors affecting green house technology
07
07
Q.5 Describe in detail:-
Procedure of ovary culture 05
Applications of hydroponics
Continuous batch culture
05
04
Q.6 Describe in detail:
Technique of hydroponics 05
Objectives of plants tissue culture 05
Applications of callus culture 04
Q.7 Any three:- 14
Sterilization methods in tissue culture
Macronutrients used in MS media
Different media used in tissue culture
Somatic embryogenesis