M.Sc. (Botany) (Semester IV) (CBCS) Examination, 2017
Plant Tissue Culture and Green House Technology and Hydroponics
Day Date: Friday, 21-04-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
N.B. Q.1 is compulsory.
Attempt any two questions from Q. 3 and 4.
Attempt any two questions from Q. 6 and 7.
Figures to the right indicate full marks.
Attempt total five questions.
Q.1 Choose correct alternatives 14
Transfer of a part of old culture to new culture vessel is known as
Subculture Inoculation
Reculture None of these
Differentiation of callus into plant parts is known as
Embryogenesis Morphogenesis
Embryoid formation Totipotency
Pollen embryoids were discovered by
Konal and Natraja Guha and Maheshwari
Skoog and Miller Helperin and Wetherell
Hardening is induced by keeping plantlets under
High light intensity and low humidity
Low light intensity and low humidity
Low light intensity and high humidity
High light intensity and high humidity
For maximum illumination, the direction of greenhouse should be
North to south East to West
South east to North east Both a and b
Tissue culture technique can produce indefinite number of new
plants from a small parental tissue. The economic importance of
this technique is in raising
Variants through picking up somaclonal variation
Genetically uniform population of an elite species
Homozygous diploid plants
Development of new species
Rock wool is the most probably widely used medium in
hydroponics, which is obtained from
Fossil remains Basalt rock
Page 2 of 2
Volcanic rock All of these
Development of shoot and root in tissue culture is determined by
Cytokinin to auxin ratio Enzymes
Plant nutrients Temperature
Which country has developed advanced hydroponics technology
due to its arid climate?
Sri Lanka UAE USA Israel
10) Who discovered that morphogenesis in tissue culture is
controlled by hormones.
Muir et. al. Vasil and Hilderbrandt
Skoog and Miller Helperin and Wetherell
11) Cell suspension culture is agitated at rpm of
120 50 160 170
12) The concept of cellular totipotency was given by
Carlson et. al. Steward
Barski et. al. Vasil and Hilderbrandt
13) In greenhouse, the heat treatment is given to soil to remove used
seeds at the temperature
60 oC 82.2 oC 37.8 oC 54.4 oC
14) Which of the following hydro gels have been used for
encapsulation of hydrated somatic embryos?
Sodium and potassium alginate
Carageenan and Gel-Rite
Sodium pectate and Agar
All of these
Q.2 Define micropropagation? Describe it in detail by using axillary buds.
Comment on its applications.
14
Q.3 Describe concept of cellular totipotency
Give an account of cell suspension culture with its significance.
07
07
Q.4 Macronutrients
Fertilizers in greenhouse
07
07
Q.5 Describe in brief:
Factors affecting another culture 07
Management of green house 07
Q.6 Write brief notes on:
Fumigation in plant tissue culture room. 07
Different growth media used in hydroponics 07
Q.7 Write short notes on any three of the following: 14
Embryo rescue
Haploid plants
Significance of greenhouse
Factors influencing morphogenesis