M.Sc. DEGREE EXAMINATION, APRIL 2016
Biotechnology
LAB V — RECOMBINANT DNA TECHNOLOGY
(CBCS 2013 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 2 20)
Answer all questions.
All questions carry equal marks.
1. CTAB
2. EtBr
3. Star activity
4. Unit of restriction enzyme
5. Hot start PCR
6. l6S rDNA
7. SYBR Green
8. RNase H
9. APS
10. Chromogenic substrate.
Sub. Code
501304
RW-10971
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Wk 9
Part B 5 25)
Answer all questions Choosing either or
11. Describe the principle of plasmid DNA isolation by
alkaline lysis method.
Or
Derive the Beer -Lambert law.
12. Explain the screening of library by southern
blotting.
Or
Give an account on sub-cloning in plasmids.
13. Explain briefly the principle of T/A cloning vector.
Or
Elaborate on site directed mutagenesis principles.
14. Write the composition of worm lysis buffer.
Or
Explain the mechanism of recombinant protein
expression in E.coli.
15. Write briefly the role of each chemical components
in SDS-PAGE.
Or
Describe the principles of purification of
recombinant protein.
Part C 10 30)
Answer any three questions.
16. Write briefly the principle of genomic DNA isolation from
plant and animal cells.
17. Describe the construction of genomic DNA library using
phage vectors.
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Wk 9
18. Write briefly various types of DNA finger printing
methods and its applications.
19. Write a detailed note on principle and applications of real
time PCR.
20. Define hybridisation. Add notes on principles,
methodology and applications of Western blotting.