M.Sc. DEGREE EXAMINATION, APRIL 2018
Second Semester
Biochemistry
BIOTECHNOLOGY
(CBCS 2017 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 X 2 20)
Answer all questions.
1. What is Nested PCR?
2. What are the different methods of sterilization?
3. What is a recombinant vaccine?
4. What is bacterial transformation?
5. Write the principle of electrophoresis.
6. What is a Fosmid?
7. Comment on viral vectors.
8. Define the term facultative anaerobe. Give an example.
9. What is the molecular defect in cystic fibrosis?
10. Write a note on abzymes.
Sub. Code
7MBC2C3
AFS-0593
2
wk 16
Part B X 5 25)
Answer all questions, choosing either or
11. Write a short note on DNA vaccines.
Or
What are monoclonal antibodies? Enumerate on
their applications.
12. How is the insert size and orientation determined in
molecular cloning?
Or
Discuss on the different types of gene cloning
vectors.
13. Discuss on various types of bioreactors.
Or
Discuss briefly on the production of L-glutamic acid
by microbial fermentation.
14. Explain the treatment approach of hemophilia with
gene transfer.
Or
Write a note on Alzheimer's disease biomarkers.
15. Give an overview on polyacrylamide gel
electrophoresis.
Or
Discuss on the principle and applications of
polymerase chain reaction.
AFS-0593
3
wk 16
Part C 10 30)
Answer any three questions.
16. Describe the general approach and basic process of gene
therapy.
17. Discuss on the production of industrial alcohol by malt
fermentation.
18. Explain in detail the synthesis and application of human
insulin from recombinant DNA.
19. What is cloning? Discuss in detail the process of
molecular cloning.
20. Discuss the principle, method and applications of
southern blotting.
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