M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
Second Semester
Biochemistry
BIOTECHNOLOGY
(CBCS 2014 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. Define phagemids.
2. Explain shuttle vectors.
3. What is the role of ethidium bromide in agarose gel
electrophoresis?
4. What is the use of northern blotting?
5. Define haemophilia.
6. Explain the use of ribozmes.
7. Give an examples of plant edible vaccines.
8. What are abzymes?
9. Explain the use of fermentro.
10. What are the substrates used for the production of SCP?
Sub. Code
4MBC2C3
AFF-4936
2
Ws8
Part B x 5 25)
Answer all questions.
11. Describe pBR322 vector.
Or
What are expression vectors?
12. Give an account on PAGE.
Or
Write about reverse transcription.
13. Explain the gene therapy for AIDS.
Or
How will you identify tuberculosis using molecular
diagnosis?
14. What are the applications of interferons?
Or
Explain the production and applications of
recombinant vaccines.
15. Give an account on sterilization technique.
Or
Explain the industrial production of ethanol.
AFF-4936
3
Ws8
Part C x 10 30)
Answer any three questions.
16. Describe the genetic map of YACs.
17. Give an account on southern blotting.
18. Explain the gene therapy for adenosine deaminase
deficiency.
19. What is MAbs? Elaborately discuss its applications.
20. Explain the types of bioreactors.
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