M.Sc. DEGREE EXAMINATION, APRIL 2016
Biotechnology
Lab I — ANALYTICAL BIOCHEMISTRY
(CBCS 2013 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 2 20)
Answer all questions.
All questions carry equal marks.
1. Colorimetry.
2. Beers law.
3. Km.
4. SGOT.
5. Buffer.
6. HPTLC.
7. Extra cellular enzymes.
8. Downstream processing.
9. Regression.
10. ANOVA.
Sub. Code
501105
RW-10968
2
Wk 10
Part B 5 25)
Answer all questions choosing either or
11. Write short notes on Spectro florimetry.
Or
How will you determine a carbohydrate by using
DNSA method?
12. Derive the Michaelis Mention equation.
Or
Write notes on estimation of blood glucose and
determination of fasting, post-prandial and random
sugar.
13. How will you determine the pI values of amino
acids?
Or
How will you separate amino acids by using paper
chromatography?
14. Write notes on ion exchange chromatography.
Or
Describe about dialysis method.
15. List the application of 2D gel electrophoresis.
Or
Write notes on measures of central tendency.
RW-10968
3
Wk 10
Part C 10 30)
Answer any three questions.
16. How will you determine protein by using Lowry's and
Bradford's method?
17. Explain the clinical significance and essay of enzymes
ALP, Amylase and Lipases.
18. Derive Henderson-Hasselbach equation and explain how
will you evaluate pKa values in acid-base titrations.
19. Write notes on
ultra filtration
gel permeation chromatography.
20. Discuss in detail about radioactive labeling and
measurement of radio activity.