M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
First Semester
Biochemistry
ANALYTICAL BIOCHEMISTRY
(CBCS 2014 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. What are the composition of Riger solution?
2. Give the principle of Rate zonal centrifugation.
3. Write the principle of electrophoresis.
4. What is chromatography?
5. Write short note on UV. Visible spectroscopic.
6. What is spectroscopy?
7. Draw the structure of dimethyl POPOP.
8. What is radioisotopes?
9. Define crystallization.
10. Give the short note on positron emission.
Sub. Code
4MBC1C2
AFF-4930
2
Sp 6
Part B x 5 25)
Answer all questions, choosing either or
11. Give an account on cell sorting.
Or
Describe differential centrifugation.
12. Give an account on agarose gel electrophoresis.
Or
Describe the principle, method and applications of
ion exchange chromatography.
13. Briefly explain the principle, instrumentation and
applications of ESR.
Or
Explain circular Dicrosim spectroscopic method.
14. Compare alpha, beta and gamma rays.
Or
Describe beta emission.
15. Elaborate the immobilization techniques.
Or
Discuss MALDI TOF techniques.
AFF-4930
3
Sp 6
Part C x 10 30)
Answer any three questions.
16. How will you determine the molecular weight by
sedimentation velocity and sedimentation equilibrium
methods?
17. Discuss in detail about GCMS.
18. Describe principle, instrumentation and applications of
NMR.
19. Explain in detail about detection and measurement of
radioactivity by scintillation counting.
20. What do you understand about DNA sequencing?
Elaborate it.
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