M.Sc. DEGREE EXAMINATION, APRIL 2018
First Semester
Bio-Chemistry
ANALYTICAL BIOCHEMISTRY
(CBCS 2017 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 X 2 20)
Answer all the questions.
1. What is cell sorting?
2. Differentiate SV and SE in molecular weight
determination.
3. What is hydroxyapatite?
4. What is starch gel electrophoresis?
5. What is the principle of PAS?
6. Why is a magnet used in ESR?
7. What are radioactive tracers?
8. How is radioactivity measured?
9. What is the principle of XRF?
10. Define proteomics.
Sub. Code
7MBC1C2
AFS-0587
2
ws2
Part B X 5 25)
Answer all questions, choosing either or
11. Elaborate on the different types of homogenization
techniques.
Or
Explain the principle and applications of
ultracentrifuge.
12. Discuss on the role of TLC in compound separation.
Or
Elaborate on the technique which separates
molecules based on size.
13. Discuss on ORD.
Or
Give a detailed account on NMR.
14. What are the safety measures to be employed while
handling radioisotopes?
Or
Write in detail on the isotopes commonly used in
biochemical studies.
15. Discuss in detail on DNA sequencing.
Or
Explain the biological applications of protein
crystallization.
AFS-0587
3
ws2
Part C X 10 30)
Answer any three questions.
16. Discuss in detail on density gradient centrifugation.
17. Elaborate on the principle and applications of IEF.
18. Explain the principle and applications of UV-Vis
spectroscopy.
19. Explain the applications of autoradiography.
20. Write in detail on MALDO-TOF-TOF.
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