B.Pharm. (Semester VII)(CGPA) Examination, 2018
PHARMACEUTICAL ANALYSIS V
Day and Date Thursday, 13-12-2018 Max. Marks 70
Time 10.30 a.m. to 1.30 p.m.
1. Multiple Choice Questions (15 ×1=15)
Pre-washing step in HPTLC is done by using
Water Ethanol
Methanol Benzene
technique where separation of sample mixture was carried out
by continuous addition of mobile phase.
Elution Frontal analysis
Displacement analysis None of these
are not separated by gel chromatography.
Racemic mixtures Enantiomeric mixtures
Isomers All of these
The tR′ =tR−tM is an equation of
Adjusted retention time Retention time
Adjusted retention volume Retention volume
Derivatization procedures in HPLC are carried for
To allow chromatography of compounds
To improve resolution
To improve sensitivity of the method
All of these
mechanism is involved in paper chromatography.
Adsorption Partition
Ion exchange Affinity
Calcium sulphate is mixed with silica gel which acts as a in
TLC.
Excipients Flowing agent
Binder Additive
Seat
No. Set P
Set P
SLR-TV 46 *SLRTV46*
of ion exchange resin undergo exchange of ion with sample
solution ion.
Fixed ion Counter ion
Both A and B Movable ion
is an application of ion exchange chromatography.
Softening of hard water Demineralization of water
Both A and B Separation of racemic mixture
10) In gel chromatography, stationary phase is prepared for packing in column
by using
Mobile phase
Solvent different than mobile phase
Both A and B
None of these
11) Electron capture detector is used for detecting samples having
functional groups.
Electron donating Electron withdrawing
Double bond All of these
12) is used as column packing material for guard column in
HPLC.
Non porous 50% Porous particle
Pellicular particle Totally porous
13) Non fluorescent solutes can be detected by fluorescence detector of HPLC
by reacting with
Ethyl chloride Methyl chloride
Thionyl chloride Dansyl chloride
14) samples are separated by gas chromatography.
Volatile and thermolabile Volatile and thermostable
Non volatile and thermolabile Non volatile and thermostable
15) Which of the following detector destroy the separated component by gas
chromatography
Flame ionization Thermal conductivity
Electron capture All of these
2. Answer any five of the following questions.
Explain with suitable diagram operational technique of adsorption column
chromatography.
Set P
*SLRTV46* SLR-TV 46
Define and classify chromatography.
Explain in detail on steps involved in HPTLC.
Explain with suitable diagram principle of size exclusion chromatography.
Enlist its applications.
define the terms retention time, retention volume, isocratic elution, gradient
elution and Rf value.
What is Gas chromatography Give its types and limitations.
3. Answer any three of the following questions. (3×10=30)
Explain with suitable diagram instrumentation of HPLC.
Write on any four detectors used in Gas chromatography.
Write in detail on Ion exchange chromatography.
What is paper chromatography Write on different papers used for it. Give
difference between TLC and paper chromatography.