M.Sc. DEGREE EXAMINATION, APRIL 2016
Second Semester
Bioinformatics
GENETICS AND GENETIC ENGINEERING
(CBCS 2013 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 2 20)
Answer all questions.
1. Write about physical properties of DNA.
2. Define Mutation.
3. What do you mean by lytic cycle?
4. Define the nature of plasmids.
5. Name any two restriction enzymes.
6. What is the role of kinases?
7. Define PCR.
8. What do you mean by cDNA library?
9. Explain mutagenesis.
10. Explain the principle of RFLP.
Sub. Code
502201
RW-10864
2
wk12
Part B 5 25)
Answer all questions.
11. Discuss Mendel law of inheritance.
Or
Explain about linkage and crossing over.
12. What are the differences between prokaryotic and
eukaryotic vectors?
Or
Discuss about YAC and BAC vectors.
13. Explain types of restriction enzymes.
Or
What are Blunt and sticky ends?
14. Explain the steps involved in cloning.
Or
Discuss about construction of cDNA libraries.
15. Write about site directed mutagenesis.
Or
Write about various applications of western
blotting.
Part C 10 30)
Answer any three questions.
16. Explain the process of recombination and transformation.
17. Discuss in detail about specialized vectors.
RW-10864
3
wk12
18. Give an outline of rDNA technology.
19. Discuss about different sequencing methods.
20. Discuss in detail about applications of genetic
engineering.