M.Sc. DEGREE EXAMINATION, APRIL 2016
Second Semester
Biotechnology
Lab IV — IMMUNOTECHNOLOGY
(CBCS 2013 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 2 20)
Answer all questions.
All questions carry equal marks.
1. Isotyping.
2. Aminopterin.
3. Incomplete antibodies.
4. Define agglutination inhibition.
5. Role of HRP in ELISA.
6. Direct staining.
7. What is knockout?
8. PVDF.
9. CO-FISH.
10. Autoradiography.
Sub. Code
501205
RW-10862
2
Wk 11
Part B 5 25)
Answer all questions choosing either or
11. Write in detail about different routes of injection in
mice.
Or
Give an account on monoclonal antibodies in clinical
diagnosis.
12. Explain the methods of agglutination.
Or
Illustrate the principle of Ouchterlony
immunodiffusion.
13. Describe the principles of blood grouping.
Or
Write a note on flow cytometry.
14. List out the differences between transient and
stable transfection methods.
Or
Describe the basic techniques used for prevention of
contamination in mammalian cell lines.
15. Explain the principle of ELISA with suitable
diagram.
Or
Write down the applications of in situ localization.
RW-10862
3
Wk 11
Part C 10 30)
Answer any three questions.
16. Write briefly the protocol of immunization to raise
antibodies.
17. Give an account on Immuno histochemical staining.
18. Discuss the rare variants of Rh. What is their clinical
significance?
19. Elucidate the expression of C. elegans immunoglobulin
genes by RT-PCR.
20. Describe the principle and applications of immune
electron microscopy.