M.Sc. DEGREE EXAMINATION, APRIL 2017
Second Semester
Biotechnology
Lab IV IMMUNO TECHNOLOGY
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all the questions.
1. Immunoglobulin
2. HAT medium.
3. Hemagglutination.
4. Name two counterstain used in IHC staining.
5. Rh factor.
6. Fluorophore.
7. Write about Microcarrier and its applications.
8. Write any two human cell lines and its applications.
9. ELISPOT.
10. Write the role of secondary antibodies in Western
blotting.
Sub. Code
501205
RW-172
2
Sp 6
Part B 5 25)
Answer all the questions, choosing either or
11. Brief the applications of monoclonal antibodies.
Or
Briefly explain the method of bleeding and serum
collection.
12. Describe the types of antigen-antibody reactions.
Or
Write short note on precipitin ring test.
13. Describe the apoptosis detection by DAPI.
Or
Describe the identification and enumeration of
lymphocytes by FACS.
14. Explain how the C.elegans immunoglobulin gene
expression monitored by RT-PCR.
Or
Enumerate the types of cell culture media.
15. List out the differences between the FISH and
GISH techniques.
Or
Give an account on immune fluorescence
microscopy.
RW-172
3
Sp 6
Part C x 10 30)
Answer any three questions.
16. Explain the production of monoclonal antibodies.
17. Describe the principle and applications of
immunoelectrophoresis with suitable diagrams.
18. Briefly discuss about the ABO blood group system and its
applications.
19. Give an account on cell synchronization and its types.
20. Explain the principle and applications of western
blotting.
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