M.Sc. DEGREE EXAMINATION, APRIL 2016
Second Semester
Biotechnology
Lab III — MOLECULAR GENETICS
(CBCS 2013 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 2 20)
Answer all questions.
All questions carry equal marks.
1. Allele.
2. Define plaques.
3. What is meant by silent mutation?
4. What is known as transversion?
5. Frederick Griffith.
6. Horizontal gene transfer.
7. Define electroporators?
8. Define prophage.
9. What is C-value?
10. What you meant by P element?
Sub. Code
501204
RW-10861
2
Wk 11
Part B 5 25)
Answer all questions, choosing either or
11. Elucidate the one step growth curve using T even
phage.
Or
Write an account on microinjection.
12. Comment on site directed mutagenesis.
Or
Define mutagen. List out the different types of nonalkylating
mutagens.
13. Define triparental mating and write its mechanism.
Or
Write down the principle of chemical method
competent cell preparation.
14. Comment on genetic mapping.
Or
Give a brief note on applications of bacteriophages.
15. Explain the mechanism of insertional inactivation.
Or
Write down the applications of transposons.
RW-10861
3
Wk 11
Part C 10 30)
Answer any three questions.
16. Calculate the phage and bacterial concentrations after
various numbers of bacterial generations.
17. Define mutagenesis. List out common chemicals used in
experimental genetics to induce mutation.
18. Describe the mechanism of bacterial transduction.
19. Compare and contrast the mechanisms of generalised and
specialised transduction.
20. Define transposons. Discuss the different types of
transposons.