M.Sc. (Biotechnology) DEGREE EXAMINATION,
APRIL 2017
Second Semester
LAB III MOLECULAR GENETICS
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all the questions.
All questions carry equal marks.
1. Genetic markers.
2. Define Phage.
3. Mutagens.
4. Auxotroph.
5. Electroporation.
6. Conjugation.
7. bacteriophage.
8. Transduction.
9. Transposon.
10. Plasmid DNA.
Sub. Code
501204
RW-074
2
Sp 5
Part B 5 25)
Answer all the questions, choosing either or
11. Elucidate the one step growth curve using T even
phage.
Or
Write a note on single colony isolation.
12. Comment on site directed mutagenesis.
Or
Describe the Ames test and its uses.
13. How will you isolate of plasmid DNA from bacteria,
describe the methodology?
Or
Write down the principle of competent cell
preparation.
14. Comment on transduction.
Or
Applications of bacteriophages.
15. Explain different types of transposons.
Or
Write down the applications of transposons.
RW-074
3
Sp 5
Part C 10 30)
Answer any three questions.
16. Discuss about single colony isolation and how will you
check it using genetic markers.
17. Explain about mutagenesis and chemicals used to induce
mutations.
18. Explain the techniques Microinjection and
Electroporation.
19. Discuss about transduction and elaborate its types.
20. Discuss about transposons and its types.
——————