M.Sc. DEGREE EXAMINATION, APRIL 2016
Second Semester
Zoology
Elective ANIMAL CELL CULTURE TECHNOLOGY
(CBCS 2014 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 2 20)
Answer all questions.
1. Draw the structure of a animal cell.
2. Primary cell lines.
3. Types of media used in animal cell culture.
4. FBS and its role.
5. Role of trypsin EDTA in cell culture.
6. Name any four human cancerous cell lines.
7. Micromanipulation.
8. Embryonic Stem Cells.
9. Apoptosis.
10. Knock out technology.
Sub. Code
509510
RW-10839
2
Sp 5
Part B 5 25)
Answer all the questions, choosing either or
All questions carry equal marks.
11. Explain in detail about the Animal cell culture
media.
Or
Briefly describe the equipments required for animal
cell culture.
12. Explain in detail about Serum Free Media.
Or
What is the role of CO2 in animal cell culture?
13. Discuss the methods to measure cytotoxicity.
Or
Write about the maintenance of cell culture.
14. Mention about the applications of animal cell
culture.
Or
Write note on cell transformation.
15. Write notes on cell cloning and fusion.
Or
Comment on three dimensional culture and tissue
engineering.
RW-10839
3
Sp 5
Part C 10 30)
Answer any three questions.
All questions carry equal marks.
16. Differentiate the primary and established cell line
cultures.
17. Write the advantages and disadvantage of serum and
serum free media.
18. Describe the basic techniques of mammalian cell culture
in in vitro condition.
19. Write notes on cell culture based vaccines.
20. Describe the hybridoma technology.