M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
Third Semester
Zoology
Elective ANIMAL CELL CULTURE TECHNOLOGY
(CBCS 2014 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. Cell lines
2. Balanced salt solution
3. Metabolic functions of culture medium
4. Role of carbon dioxide culture medium
5. Disaggregation of tissue
6. Cell separation
7. Synchronization
8. Stem cell culture
9. Cell death
10. Hybridoma technology.
Sub. Code
4MZO3E2
AFF-4925
2
sp4
Part B 5 25)
Answer all questions.
11. Explain about the Primary and established cell line
culture.
Or
Define the structure and organization of animal cell.
12. Briefly explain the chemical, physical and metabolic
functions of culture medium.
Or
Describe Role of serum and supplement in culture
medium.
13. Briefly explain the measurement of viability and
cytotoxicity.
Or
Describe basic techniques of mammalian cell
culture in vitro.
14. Explain about cell cloning and manipulation.
Or
Write a note on the application of embryonic stem
cells.
15. Explain about tissue engineering.
Or
Explain the mass cell cultivation.
AFF-4925
3
sp4
Part C 10 30)
Answer any three questions.
16. Briefly explain about introduction to the balanced salt
solution and simple growth medium.
17. Write note on the role of serum, co2 and Protein free
defined media with their application.
18. Explain about the Maintenance of cell culture and cell
separation.
19. Write scaling up and application of animal cell culture.
20. Explain about the cell cloning and fusion.
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