FACULTY OF PHARMACY
B. Pharmacy 4/4 II Semester (Main) Examination, March 2014
Subject: Pharmaceutical Biotechnology
Time 3 Hours Max. Marks: 70
Note: Answer all questions. All questions carry equal marks.
1 Describe the steps involved in rDNA Technology.
What are Restriction Endonucleases and DNA ligases.
OR
Explain the production of insulin by rDNA technology.
2 Explain the methods of strain development.
OR
Explain the production of amalyses by fermentation technology.
3 Explain manufacture, standardization, storage and specific applications of
pertusis vaccine.
OR
Explain the production of BCG and polio vaccines.
4 What are plasma substitutes? Explain in detail the production of Dextran.
OR
Explain the extraction and isolation of pure substance from Adrenal and
Pancreas glands.
5 Explain types and methods of biotransformation.
OR
Explain the methods of immobilization of enzymes with few industrial
applications.