FACULTY OF PHARMACY
B. Pharmacy 4/4 II-Semester (Main) Examination, April 2018
Subject Pharmaceutical Biotechnology
Time 3 Hours Max. Marks: 70
Note: Answer all questions. All questions carry equal marks.
1 Define rDNA technology. Write the steps involved in the manufacture of Insulin by
rDNA technology.
OR
Write about the following (any two)
Gene expression of rDNA
Interferons
(iii)Restriction Endonucleases
2 Give an account of microbiological assay of antibiotics as per I.P.
OR
Briefly explain any two of the following:
Maintenance of Industrial microorganism.
Batch and continuous fermentation
Immobilization of Enzymes
3 Write the preparation and purified form of diphtheria vaccine.
Explain about immunodiagnostic preparation for TB
OR
Give an account of viral vaccines.
4 Explain the preparation of dried human plasma. Explain its advantages of whole human blood.
OR
What are requirements of an ideal plasma substitute? Explain the preparation of dextran.
5 Give an account of microbial transformation of steroids.
OR
Explain animal cell culture techniques and their applications.