FACULTY OF PHARMACY
B. Pharmacy 4/4 I Semester (Suppl.) Examination, April 2017
Subject: Pharmaceutical Analysis II
(Instrumental Methods of Analysis)
Time: 3 Hours Max.Marks: 70
Note: Answer all questions. All questions carry equal marks.
1 Write about different properties of electromagnetic radiation. 5
Write the theory and principles of UV spectroscopy. 5
Explain the concept of chromophore and auxochrome. 4
OR
State and explain Beer's law and write it's deviations in applicability. 7
What is absorption maximum? Write about different factors affecting max λ of organic
compounds. 7
2 Write the principles of IR spectroscopy. 4
Explain different types of molecular vibrations in IR spectroscopy. 6
Write about finger print region and functional group region in IR spectrum. 4
OR
Write about different sample handling techniques in IR analysis of organic
compounds. 7
Describe different types of detectors used in IR spectrophotometers. 7
3 Explain the following:
Radiative and non-radiative process 7
ii) Describe the different of components of NMR spectrometer with neat labeled
diagram. 7
OR
Explain the principles of fluorescence and phosphorescence phenomena. 4
Write about different ionization techniques in mass spectroscopy. 10
4 Describe different ion selective electrodes used in potentiometric titrations. 7
Write short notes on nepheloemetry and turbidometry. 7
OR
Write about different types of amperometric titrations. 7
Write the advantages and applications of conductometric titrations. 7
5 Explain the principles of HPLC technique. 4
Write about column packing materials and mobile phase solvents used in HPLC
technique. 6
Compare HPLC and HPTLC techniques in applications. 4
OR
Write the theory and principles of electrophoresis technique. 7
Write about different types of detectors used in gas chromatography. 7