FACULTY OF PHARMACY
B. Pharmacy 4/4 I Semester (Suppl.) Examination, April 2018
Subject: Pharmaceutical Analysis II
(Instruments Methods of Analysis)
Time: 3 Hours Max.Marks: 70
Note: Answer all questions. All questions carry equal marks.
1 Write about different properties of electromagnetic radiation. 5
Write the theory and principles of UV spectroscopy. 5
Explain the concept of chromophore and auxochrome. 4
OR
Describe different methods for quantitative analysis of single component samples by
spectrophotometry. 10
State and explain beer lamberts law and deviations in its applicability. 4
2 Explain Hook's law and intensity of absorption bands in IR spectroscopy. 6
Describe the interpretation of IR spectra of simple organic compounds. 8
OR
Explain the finger print region and characteristic absorptions of any five functional
groups by drawing a schematic IR spectrum. 7
Explain different sample handling techniques used in IR spectroscopy. 7
3 Explain the principles of fluorescence and phosphorescence phenomena with help
of Jablonski diagram. 7
Write about different ionization techniques in mass spectroscopy. 7
OR
Explain the following:
Factors influencing intensity of fluorescence 6
ii) Radiative and non-radiative process 4
iii) Chemical shift and spin-spin coupling. 4
4 Explain the following:
Nernst equation and calculation of cell potential. 5
ii) Molar conductance and specific conductance 5
iii) Effect of dilution on conductance. 4
OR
Describe different ion-selective electrodes used in potentiometric titrations. 7
Write short notes on Nehelometry and turbidometry. 7
5 Give the description and working of HPLC with help of neat labelled diagram. 14
OR
Explain the principles and applications thin layer chromatography. 5
Explain about any three detectors used in gas chromatography. 9