M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
Third Semester
Botany
PLANT BIOTECHNOLOGY
(CBCS 2011 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all the questions.
Write short notes on
1. DNA ligase.
2. Primer.
3. Disarmed plasmid.
4. Selectable marker.
5. Filter sterilization.
6. Caulogenesis.
7. Biohydrogen.
8. Pencillin.
9. Copyright.
10. Trade secret.
Sub. Code
1MBO3C3
AFC-10549
2
Wk 11
Part B x 5 25)
Answer all the questions, choosing either or
11. What are different types of restriction
endonucleases?
Or
Briefly explain the role of plasmid vectors in
bacterial cloning with pBR322 as an example.
12. Describe the organization of Ti plasmid with special
reference to its T-DNA and Vir region.
Or
What is co-integrate vector system? Explain the
same with reference to Ti Plasmid.
13. Write a general account of organogenesis.
Or
Explain the protocol for anther and pollen culture.
14. Describe the process of vinegar production.
Or
Explain the principle, construction and production
of biogas from biogas plant.
15. Explain in detail the basics of IPR.
Or
Explain the Bt-gene controversy.
AFC-10549
3
Wk 11
Part C x 10 30)
Answer any three questions.
16. Describe the various steps of PCR and its applications.
17. Explain the steps involved in the regeneration of
transgenic plants through Agrobacterium mediated gene
transfer.
18. Explain the different techniques involved in isolation in
protoplast.
19. Describe the production of citric acid.
20. What are the opposing views on the introduction of GM
crops?
————————