M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
Third Semester
Botany
PLANT BIO-TECHNOLOGY
(CBCS 2014 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. Complementary DNA.
2. Cosmids.
3. T-DNA.
4. Micro injection.
5. Filter sterilization.
6. Callus.
7. In situ Bioremediation.
8. Methanogenesis.
9. TRIPS.
10. Biosafety.
Sub. Code
4MBO3C2
AFF-4906
2
Wss
Part B x 5 25)
Answer all questions, choosing either or
11. What are the different kinds of plasmids and what
is their role in genetic engineering?
Or
Define cDNA. Describe the procedure for preparing
for a cDNA library.
12. Describe the structure of Ti plasmid.
Or
Explain the role of Agrobacterium tumefaciens in
plant genetic engineering.
13. Describe the different methods of sterilization.
Or
Write short notes on artificial seeds.
14. Describe the production of citric acid.
Or
Define Bt endotoxin. Explain its mechanism in the
development of insect resistance in transgenic
plants.
15. Give an account of Patent.
Or
Comment on copyrights.
AFF-4906
3
Wss
Part C x 10 30)
Answer any three questions.
16. Explain the principle and working mechanism of
Polymerase chain reaction. Add a note on its applications.
17. Explain the direct gene transfer methods in plants.
18. Explain the direct and sequential enzymatic treatment to
isolate protoplast.
19. Describe about the strategies employed for genetically
engineered plants for herbicide resistance.
20. Discuss case studies related to biopiracy.
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