M.SC. Genetics (Semester IV) (CBCS) Examination, 2017
ANIMAL CELL CULTURE
Day Date: Wednesday, 26-04-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions Section-I is compulsory.
From Section -II attempt any four.
All questions carry equal marks.
Figures to right indicate full marks.
Draw neat and labeled diagrams wherever
necessary.
Q.1 Rewrite the following sentence by using correct alternative. 07
HeLa cell line is derived from cell line.
Stomach Cervical Lung Blood
Which of the following behavior not shown by normal cell in
culture?
Contact inhibition Monolayer formation
Uncontrolled cell division Anchorage dependent
Hybridoma technique used for monoclonal antibodies
discovered by
Milstein Harrison Carrel Skoog
Isolation of lymphocyte from blood is carried out by using

PBS Ficol Hypaque EMEM BSS
Laminar air flow platform sterilized by using.
70% ethanol 100% ethanol
50% ethanol 30% ethanol
chemical method used to get all the cells in same phase
of growth in culture.
Electrophoresis Metabolic Inhibitor
GLC Centrifugation
is indirect method of cell monitoring.
Protein estimation Hemocytometry
Coulter counter Viability count
Page 1 of 2
Answer the following terms: 07
Define serum.
Describe in brief laminar air flow.
Explain in brief karyotyping.
Write a note on Sterilization of Glasswares.
Explain in brief animal cell line.
Define Senescence.
Explain in brief cell repositories.
SECTION-II
Q.2 Describe physiological properties of media. 14
Q.3 Give details of applications of animal cell culture. 14
Q.4 Discuss in detail characteristics of culture cells 14
Q.5 Answer any TWO of the following 14
Describe sterilization practices in ACT
Write a note on identifications of specific cell lines.
Explain in brief BSS.
Q.6 Answer any TWO of the following: 14
Write a note on morphology of culture cells.
Write a note on culture based vaccine.
Discuss instruments used in ATC