M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Genetics
ANIMAL CELL CULTURE
Time: 2½ Hours Max. Marks: 70
Instructions: Part-I, question 1 is compulsory.
Attempt any four questions from Part II
All question carry equal marks.
Draw neat and labeled diagrams wherever necessary.
Figures to the right indicate full marks.
PART I
Q.1 Rewrite the sentence after choosing correct answer from the given
alternatives:
07
In primary culture, cell divides to give different type of cells by
process.
Proliferation Differentiation
Cultivation Initiation
is a physical method used to get all the cells in same
phase of growth in culture.
TLC HPLC
Cell size sedimentation Electrophoresis
Normal cell divide a limited number of time then die is a
predetermined event known as
Differentiation Proliferation
Cell density Senescence
Milstein discovered technique used for monoclonal
antibodies production.
Cell counting Di-deoxy sequencing
Protoplast fusion Hybridoma
Isolation of lymphocyte from blood is carried out by using
PBS Ficol hypaque
EMEM BSS
Laminar air flow platform sterilized by using
70% ethanol 100 ethanol
50 ethanol 30% ethanol
In year carrel designed suitable flask for routine animal
cell culture.
1915 1923
1925 1924
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SLR-VK-324
Q.1 Define the following terms. 07
Viral contaminants
Inverted microscope
Labeled antibodies
Cell counting
Define cell cycle
Define monoclonal antibodies
Plasminogen activators
PART II
Answer any four of the following:
Q.2 Describe applications of animal cell culture. 14
Q.3 Give details of laboratory design for animal cell culture. 14
Q.4 Discuss culture media for animal cell culture. 14
Q.5 Answer any Two of the following:
Describe Karotyping.
Write a note on analysis of cell cycle.
Explain indirect method of cell determination
14
Q.6 Answer any Two of the following: 14
Write a note on applications of transgenic animals.
Write a note on Gene therapy.
Discuss sterilization of apparatus in animal cell culture.