M.Sc. (Semester III) (CBCS) Examination Oct/Nov-2017
Botany
CYTOGENETICS, PLANT BREEDING AND GENETIC ENGINEERING
Day Date: Saturday, 18-11-2017 Max. Marks: 70
Time: 02.30 PM to 05.00 PM
Instructions: Q.1 is compulsory.
Attempt any two questions from Q. 3 and 4.
Attempt any two questions from Q. 6 and 7.
Q.1 Tick mark the right answer of following objective: 14
is/are the basis of variation in organisms.
Mutation Recombination
Both a b None of these
Viroids have
ss RNA not enclosed by protein coat
ss DNA enclosed by protein coat
ds DNA enclosed by protein coat
ds RNA not enclosed by protein coat
Trascriptionlly active chromatin tends to be get
Light stained Dark stained
Partially stained Non stained
In the lac operon system B-galactosidase is coded by
A gene X gene
Y gene Z gene
Protoplasts can be isolated by
Mechanical method Enzymatic method
Both a and b None of these
Eukaryotic genome is larger in size as compared to prokaryotic genome as
eukaryotic genome has
Introns Transposons
Satellite DNA All of these
One of the following is not the transposon
SINES LINES
LTR STR
The most commonly used molecular market for agronomic (yield) traits in
plant breeding is
RFLP QTL
RAPD AFLP
Pomato is an example of
Cybrid Somatic hybrid
Normal hybrid None of these
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SLR-MD-137
10) One of the following is not the unit of gene mapping
Map unit Centi Morgan
Crossing Over recombination frequency
11) If bacterial genome and plasmid allowed to replicate in the same manner
then
Bactericides genome replicates faster
Plasmid replicates faster
Both will take equal time for replication
None of these
12) Transposable genetic elements ware discovered by
McClintock Mendel
Meselson Morgan
13) Proteins that assist binding of RNA polymerase to the promoter region on
DNA strand are called
Transcription factor SSB protein
Sigma Factor All of the above
14) Which of the following is true regarding linkage maps? They
Can be used to pinpoint the precise physical position of a gene on a
chromosome
Are a genetic map based recombination frequencies
Require preparation of karyotypes.
Reflect the frequency of crossing over between X and Y
chromosomes
Q.2 Give the comparative account of the genome in prokaryotes and eukaryotes
with respect to structure, organization and packing.
14
Q.3 What is bioinformatics explain use of bioinformatics in major research area. 14
Q.4 What is chromosome mapping? Explain deferent types with importance. 14
Q.5 Describe in briefly. 14
Gene families
Mobile genetic elements and their significance
Q.6 Write on: 14
Site specific recombination
Somatic cell genetics
Q.7 Write short notes on any three of the following: 14
Saccharomyces cerevisae genome
Hybridoma technology
Protoplast fusion
IPR