M.Sc. (Semester III) (CBCS) Examination Nov/Dec-2018
Botany
CYTOGENETICS, PLANT BREEDING AND GENETIC ENGINEERING
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Draw a neat, well labeled, complete diagram wherever necessary.
Use of calculators, cell phones, or any other electronic gadgets is
prohibited.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
A fully formed infectious viral particle is called as
Voiriod Virusoid
Virion Capsid
Tobacco mosaic virus is
Spherical shaped Rod shaped
Cuboidal shaped Oval shaped
Proteins responsible for compact packing and winding chromosomal DNA are

Histone Nonhistones
Trypsin Serein
Type of chromatin that participates in active transcription of DNA to mRNA
products is
Heterochromatin Euchromatin
Centromere Achrcentric chromosome
Region of repetitive nucleotide sequences at each end of chromatid is
Centromere Telomere
Isomers Monomers
Euchromatin is
Lightly packed form of chromatin
Tightly packed form of chromatin
Concentrated packed form of chromatin
Elongated form of chromatin
Which of the following process occurs between DNA molecules of very similar
sequences?
Homologous genetic sequences
Site specific recombination
Non homologous recombination
Replicative recombination
Which of the following does not code for an enzyme having both helicae ad
nuclease activity?
Rec A Rec B
Rec C Rec D
Page 2 of 2
SLR-VD-103
One centiMorghan is defined as percent of the total recombination events.
1 10
0.1 0.01
10) Hormone pair required for a callus to differentiate are
Auxin and Cytiokinin Auxin and ethylene
Auxin and abscisic acid Cytokinins and giberllin
11) Emasculation is concerned with
Hybridization Clonal selection
Mass selection Pure line selection
12) Hybridomas are made by
Fusing T cells with myeloma cells
Fusing B cells with myeloma cells
Fusing T helper cells with myeloma cells
Fusing B memory cells with myeloma cells
13) Monoclonal antibodies are used in
Screening of recombinants Diagnostic kits
The treatment of many cancers All of these
14) Which of the following is not a variant of BLAST
BLASTN BLASTP
BLASTX TBLASTNX
Q.2 Answer the following:- (Any four) 08
Hybrids
Protein data bank
Importance of IPR
Regeneration of protoplast
Amplification
Write notes on. (Any two) 06
Gene conversion
Independent assortment
Physical map
Q.3 Answer the following:- (Any two) 08
Write a note on linkage group.
Explain gene mapping.
Use of bioinformatics in research
Answer the following:- (Any one) 06
Explain genome structure of plasmid.
Describe the method of protoplast isolation.
Q.4 Answer the following:- (Any two) 10
Explain hybridoma technology.
Explain site specific recombination.
Write a note on mobile genetic element.
Answer the following:- (Any one) 04
Write a note on NCBI.
Explain mechanism of crossing over.
Q.5 Answer the following:- (Any two) 14
Explain size and structure of genome in eukaryotics.
Describe chromosome mapping.
Explain in detail somaclonal variation.