Exam Details
| Subject | biotechnology | |
| Paper | ||
| Exam / Course | b. pharmacy | |
| Department | ||
| Organization | solapur university | |
| Position | ||
| Exam Date | November, 2017 | |
| City, State | andhra pradesh, solapur |
Question Paper
B.Pharmacy (Semester Examination, 2017
BIOTECHNOLOGY (CGPA Pattern)
Day and Date Monday, 11-12-2017 Total Marks 70
Time 10.30 a.m. to 1.30 p.m.
Note Figures to right indicate marks.
1. Choose the correct answer (1×15=15)
UPT kit is useful to confirm
Diabetes Pregnancy
Blood pressure Hepatitis A
reagent is used to confirm RNA.
Bile DPA FDA Safranine
discovered RNA in 1909.
Phoebus levene Rous Guarine Weismann
Identify vector mediated gene transfer technique.
Electroporation
Liposome mediated gene transfer
PEG mediated gene transfer
Agrobacterium mediated gene transfer
is used to improve blood flow during surgery.
Penicillin Dextran
Streptomycin Vitamin B12
E. coli DNA ligase enzyme has an ability to join of DNA
fragments.
Blunt ends Sticky ends Coding ends Genetic ends
Alexander Fleming discovered penicillin in
1919 1929 1939 1949
enzyme is isolated from Thermus aquaticus.
Taq polymerase Taq ligase Taq kinase Taq nuclease
Most suitable pH required for the commercial production of streptomycin
is
7 to 8 3 to 4 9 to 10 5 to 6
10) requires for cleavage of DNA molecules.
Type I restriction endonuclease
Type II restriction endonuclease
Type III restriction endonuclease
Type IV restriction endonuclease
11) Identify the device used to control constant water flow in fermentation process.
Tachometer Flow meter Rota meter Speedometer
12) Identify the substrates used for growth of anchorage dependent animal cells.
Glass Plastic Metal All of these
13) is most commonly used fusogen in conjunction with alkaline
pH and high calcium concentrations.
PEG NaNO3
PVA DMSO
14) What is the term used to define capacity of the cell to give rise to the whole
plant
PTC Xenoplantation Totipotency Protoplast
15) is most superior cryoprotectant used in cryopreservation.
PEG NaNO3 PVA DMSO
2. Answer any five of the following questions
Write a note on cosmid as vector.
Define down stream processing. Why is it to be done
Describe protoplast fusion with their applications.
Discuss liposome mediated gene transfer with their merits and demerits.
Explain historical background of biotechnology.
Write a note on trypsinization.
3. Answer any three of the following questions (3×10=30)
Explain production of streptomycin by fermentation technology.
Discuss protoplast culture with their applications.
Add a note on PCR.
Discuss production of monoclonal antibodies of hybridoma technology.
BIOTECHNOLOGY (CGPA Pattern)
Day and Date Monday, 11-12-2017 Total Marks 70
Time 10.30 a.m. to 1.30 p.m.
Note Figures to right indicate marks.
1. Choose the correct answer (1×15=15)
UPT kit is useful to confirm
Diabetes Pregnancy
Blood pressure Hepatitis A
reagent is used to confirm RNA.
Bile DPA FDA Safranine
discovered RNA in 1909.
Phoebus levene Rous Guarine Weismann
Identify vector mediated gene transfer technique.
Electroporation
Liposome mediated gene transfer
PEG mediated gene transfer
Agrobacterium mediated gene transfer
is used to improve blood flow during surgery.
Penicillin Dextran
Streptomycin Vitamin B12
E. coli DNA ligase enzyme has an ability to join of DNA
fragments.
Blunt ends Sticky ends Coding ends Genetic ends
Alexander Fleming discovered penicillin in
1919 1929 1939 1949
enzyme is isolated from Thermus aquaticus.
Taq polymerase Taq ligase Taq kinase Taq nuclease
Most suitable pH required for the commercial production of streptomycin
is
7 to 8 3 to 4 9 to 10 5 to 6
10) requires for cleavage of DNA molecules.
Type I restriction endonuclease
Type II restriction endonuclease
Type III restriction endonuclease
Type IV restriction endonuclease
11) Identify the device used to control constant water flow in fermentation process.
Tachometer Flow meter Rota meter Speedometer
12) Identify the substrates used for growth of anchorage dependent animal cells.
Glass Plastic Metal All of these
13) is most commonly used fusogen in conjunction with alkaline
pH and high calcium concentrations.
PEG NaNO3
PVA DMSO
14) What is the term used to define capacity of the cell to give rise to the whole
plant
PTC Xenoplantation Totipotency Protoplast
15) is most superior cryoprotectant used in cryopreservation.
PEG NaNO3 PVA DMSO
2. Answer any five of the following questions
Write a note on cosmid as vector.
Define down stream processing. Why is it to be done
Describe protoplast fusion with their applications.
Discuss liposome mediated gene transfer with their merits and demerits.
Explain historical background of biotechnology.
Write a note on trypsinization.
3. Answer any three of the following questions (3×10=30)
Explain production of streptomycin by fermentation technology.
Discuss protoplast culture with their applications.
Add a note on PCR.
Discuss production of monoclonal antibodies of hybridoma technology.
Other Question Papers
Subjects
- anatomy, physiology and health education – i
- anatomy, physiology and health education – ii
- biochemistry
- biochemistry – i
- biochemistry – ii
- biopharmaceutics
- biotechnology
- clinical pharmacology
- herbal technology
- human anatomy and physiology – i
- human anatomy and physiology – ii
- medicinal chemistry – i
- medicinal chemistry – ii
- medicinal chemistry – iv
- microbiology
- modern dispensing and hospital pharmacy
- novel drug delivery systems
- organic chemistry – i
- organic chemistry – ii
- organic chemistry – iii
- pathophysiology (new cbcs)
- pathophysiology and clinical biochemistry – i
- pathophysiology and clinical biochemistry – ii
- pharmaceutical analysis – i
- pharmaceutical analysis – ii
- pharmaceutical analysis – iv
- pharmaceutical analysis – v
- pharmaceutical analysis – vi
- pharmaceutical busines management
- pharmaceutical engineering
- pharmaceutical enginering
- pharmaceutical inorganic chemistry
- pharmaceutical jurisprudence
- pharmaceutical microbiology
- pharmaceutical organic chemistry – ii
- pharmaceutical organic chemistry –i
- pharmaceutics – i (new cbcs)
- pharmaceutics – i (old-cbcs pattern)
- pharmaceutics – ii
- pharmaceutics – iii
- pharmaceutics – iv
- pharmacognosy – i
- pharmacognosy – ii
- pharmacognosy – iii
- pharmacology – i (new) (cbcs pattern)
- pharmacology – ii
- pharmacology – ii (cgpa pattern)
- pharmacology – iv
- physical pharmaceutics – i
- physical pharmacy – i
- physical pharmacy – ii
- sterile dosage forms