B.Pharmacy (Semester (New CBCS) Examination, 2018
biotechnology
Day and Date Thursday, 20-12-2018 Total Marks 70
Time 10.30 a.m. to 1.30 p.m.
N.B. Figures to right indicate marks.
1. Choose the correct answer (1×15=15)
The name Kary Mullis is associated with
PAGE RFLP
SDS-PAGE PCR
harmone secreted in urine is responsible to show positive
reaction for UPT kit during pregnancy.
Progesterone Estrogen
Chorionic Cortisone
Vitamin B12 is produced from the strains of by fermentation
technology.
Streptomyces olivaceus Pseudomonas denitrificans
Clostridium butyricum All of these
is an intracellular product obtained by fermentation technology.
Penicillin Streptomycin
Cobalamine Dextran
Optimum pH maintained during the production of penicillin by fermentation
process is
4.2 to 4.8 9.2 to 10.2
6.2 to 6.8 8.2 to 8.8
Conversion of cortisone to prednisone in presence of Corynebacterium
simplex is reaction.
Dehydrogenation Reduction
Esterification Hydrolysis
P.T.O.
Set P
Most widely used chemical for protoplast fusion as fusogen is
Mannitol Sorbitol
Mannol PEG
Part of plant used for culturing is called as
Scion Explants
Stock Callus
Anchorage dependent animal cells require as support of
substrate for their normal growth.
Borosilicate glass Cotton
Wool Jute
10) Disaggregation of cells can be achieved by
Physical disruption Enzymatic digestion
Treating with chelating agents All of these
11) Aminobenzyloxymethlyl filter paper is commonly used for transfer in
blotting.
Western Southern
Northern Dot
12) PCR is a
DNA Degradation Technique DNA Amplification Technique
DNA Sequencing Technique All of these
13) In which condition electrophoresis not used
Separation of proteins Separation of amino acids
Separation of lipids Separation of nucleic acids
14) How many classes of restriction endonucleases are there
1 2
3 4
15) Monoclonal antibodies are
Heterogenous antibodies produced from single clone of plasma cells
Homogeneous antibodies produced from single clone of plasma cells
Both and
None of these
SLR-TV 36 *SLRTV36*
Set P
2. Answer any five of the following questions
Discuss scope of biotechnology with reference to biomedical field and
enzyme industry.
Explain construction of conventional fermenter with neat labeled diagram.
Describe various components of media used in plant tissue culture.
Add a note on Agarose gel electrophoresis.
Explain any one technique of gene therapy.
How do dextran produced by fermentation technology
3. Answer any three of the following questions (10×3=30)
Explain various stages involved in Down Stream Processing with their
significance.
Discuss various steps used in isolation of protoplast culture. How the plants
are to be generated from leaf protoplast
What do you mean by biotransformation Write any six bioconversion
reactions with reference to steroids.
Write a note on
Applications of PCR
Role of serum in serum containing media.