M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Genetics
GENETIC ENGINEERING
Time: 2½ Hours Max. Marks: 70
Instructions: Section I is compulsory.
Attempt any four questions from Section II
All questions carry equal marks.
Figures to the right indicate full marks.
Section I
Q.1 Rewrite the sentence after choosing correct answer from the given
alternatives:
07
Which of the following is not a restriction endonuclease
Eco RI Bam HI
Hind III DNA ligase
The set of DNAs generated by using random primers in a PCR
reaction is called
RAPD RFLP
AFLP In situ hybrids
Any DNA molecule that has ability to replicate in an appropriate host
cell, to which the desired gene are integrated for cloning is called as,
Plasmid Linker
Vector Adaptor
The virus commonly used to infect cell culture for the production of
interferon is
Corona virus Small pox virus
Sendai virus Polio virus
The transformation method that uses tungsten or gold particle coated
with DNA accelerated at high velocity is called
Gun acceleration Micro injection
Electroporation Particle gun delivery method
Agrobacterium tumefaciens is a
Gram negative soil bacterium causing crown gall disease in dicots
Gram positive soil bacterium causing crown gall disease in
monocot
Causing cancer in human
Gram negative soil bacterium causing crown gall disease in
monocot.
Introduction of recombinant DNA into bacterial cell by using electric
pulse is called
Transduction Transformation
Electroporation Replication
Page 2 of 2
SLR-VK-325
Q.1 Define the following terms. 07
Exonucleases
Define DNA probe
Transgenic animals
Chromosome walking
Edible vaccines
What is Genetic Engineering?
Cosmids
Section II
Answer any four of the following:
Q.2 What is transgenic animals? Write method of production of recombinant insulin. 14
Q.3 Discuss in detail Sanger's dideoxy method of DNA sequencing. 14
Q.4 Explain in detail RFLP add a note on its application. 14
Q.5 Answer any two of the following:
Explain production of Hepatitis B recombinant vaccine.
Describe PEG mediated microinjection method of DNA transfer.
Structure of plasmid.
14
Q.6 Answer any two of the following: 14
PCR its types
Write note on C-DNA library
Explain development of salt stress tolerant plants.