M.Sc. (Semester III) (CBCS) Examination Nov/Dec-2018
Genetics
GENETIC ENGINEERING
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Draw neat and labeled diagrams.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
What happens if a DNA molecule is treated by first Exonuclease III and then
followed by treatment with S1 nuclease?
The molecule is shortened from both the ends
The molecule is shortened only from ends
Only Exonuclease acts and S1 doesn't acts
The molecule is shortened only from en.
The vectors commonly used for sequencing human genome is
Plasmid YAC
M 13 phge
DNA solution injected directly into the cell using micromanipulator is called

Macroinjection
Micromanipulator mediated DNA delivery
Microinjection
Microfection
The set of DNAs generated by using random primers in PCR reaction is called

AFLP RT PCR
RFLP RAPD
The first engineered plasmid vector is
pSC 101 pUC 18
pBR 322 pSC 100
Introduction of DNA into cells by exposing to high voltage electric pulse is

Electrofusion Electroporation
Electrofision Electrolysis
Which of the following statement is correct with respect to exonuclease?
They only act on single stranded DNA molecules
They only act on double stranded DNA molecules
They remove a single nucleotide base at a time
They remove nucleotide bases from the middle of polynucleotide chain
Which of the following statements is correct regarding S1 nuclease?
It is obtained from E. Coli It acts on double stranded DNA
It acts on both types of strands It acts on single stranded DNA
The most popular and widely used engineered plasmid vector is
pBR 322 pUC 18
pSC 101 pBR 327
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SLR-VK-317
10) Which of the following Chemical enhances vir gene expression?
Cyanidin Acetosyringone
Glutennin Dextran
11) If DNA is digested by endonucleases in four sites giving rise to fragments of
which two are equal in length how many bands would be seen after
electrophoresis?
2 3
4 5
12) The recognition sequence for BamHI is G GATCC The represents
the cutting site. What can be inferred about the ends from it?
The ends created are double stranded
To decide about the nature of the ends more information is needed
The single stranded end is in nature
The single stranded end is in nature
13) of the following has flat/irregular Crystal shape.
CRY III(subgroup) CRYII( subgroup)
CRY IV CRY I (subgroup)
14) Monellin is a which is 3000 times sweeter than sucrose.
Aldehyde Protein
Lipid Amino acid
Q.2 Answer the following any four:-
1. Enlist the advantages applications of electroporation.
2. Define Vectors.
3. Write a note on C-DNA library.
4. Define Exonucleases endonucleases.
5. Give brief account on introduction of genetic engineering.
08
Write notes on (Any
1. Write a note on recombinant insulin.
2. Write a note on particle gun mediated gene transfer.
3. Write a note on Genomic DNA probes.
06
Q.3 Answer the following Any
1. Give detail of Direct Screening
2. Explain Edible Vaccine.
3. Discuss shuttle vector.
08
Answer the following any one
1. Describe Construction of Genomic DNA library.
2. Explain T4
DNA Ligase with mode of action.
06
Q.4 Answer the following Any
1. Discuss reverse transcriptase PCR.
2. Explain pBR 322 and pSC 101 vector.
3. Describe chemical methods used for direct DNA transfer.
10
Answer the following any one
1. Describe DNA polymerase I.
2. Write a note on fusion protein.
04
Q.5 Answer the following. (Any two) 14
Explain in detail RFLP as molecular Marker.
Describe chain termination method of DNA sequencing.
Discuss Development of Insect Resistant Plants.