Exam Details
| Subject | Separation methods | |
| Paper | ||
| Exam / Course | Post Graduate Diploma in Analytical Chemistry | |
| Department | School of Sciences (SOS) | |
| Organization | indira gandhi national open university | |
| Position | ||
| Exam Date | December, 2015 | |
| City, State | new delhi, |
Question Paper
Explain 'separation' and its objectives. Describe the scope and uses of separation methods.
Name any two extractants each containing C-0 and P 0 bonds. List the essential characteristics of diluents.
Discuss the role of masking agents in achieving selectivity.
Name any two chelating agents (along with their structures) used in solvent extraction. Draw the plot between pH and log D. How will you determine the number of molecules of a chelating agent from this plot
Explain the terms distribution constant, retention time and retention factor.
Define selectivity, separation factor and resolution of two components with retention times tx and ty, respectively. Show a chromatogram with retention times for solvents and two components as 0·73, 1·89 and 2·76 min, respectively. Explain the role of peak width in resolution.
Draw a sketch of gas chromatograph showing all the components.
List the various column development techniques and explain briefly anyone of them.
What are the two planar chromatographic methods? Explain their principle and the common parameter. In what respects do these differ from each other?
Compare the various forms of liquid chromatography i.e. classical column and HPLC using pellicular packing and microparticulate packing in terms of particle size, column diameter and its length.
What are the various criteria for the selection of mobile phase for HPLC Explain gradient elution.
Explain the autoradiography using radiotracers with a suitable example. What are its advantages and limitations?
What are the essential requirements of a detector in GC
Explain the basic features of ion exchange mechanism. What is the difference between adsorption and ion exchange?
Define resin selectivity. What are the various factors on which selectivity depends?
What are gels Describe the various properties of gels which make them useful for chromatography.
What are polyacrylamide gels? In what way are these different from Sephadex gels?
Define the distribution ratio and percent extraction E and show that 100D/ D+1.
What do you understand by capillary electrophoresis? Explain it schematically.
Explain the terms -Liquid-solid and gas-solid adsorption chromatography.
Explain dialysis and electrodialysis with an illustration for each. In what respects do these differ from each other?
8. Write brief explanatory notes on any three of the following:
Modifier
Reverse Phase Chromatography
DNA Gel Electrophoresis
Donnan Effect
Name any two extractants each containing C-0 and P 0 bonds. List the essential characteristics of diluents.
Discuss the role of masking agents in achieving selectivity.
Name any two chelating agents (along with their structures) used in solvent extraction. Draw the plot between pH and log D. How will you determine the number of molecules of a chelating agent from this plot
Explain the terms distribution constant, retention time and retention factor.
Define selectivity, separation factor and resolution of two components with retention times tx and ty, respectively. Show a chromatogram with retention times for solvents and two components as 0·73, 1·89 and 2·76 min, respectively. Explain the role of peak width in resolution.
Draw a sketch of gas chromatograph showing all the components.
List the various column development techniques and explain briefly anyone of them.
What are the two planar chromatographic methods? Explain their principle and the common parameter. In what respects do these differ from each other?
Compare the various forms of liquid chromatography i.e. classical column and HPLC using pellicular packing and microparticulate packing in terms of particle size, column diameter and its length.
What are the various criteria for the selection of mobile phase for HPLC Explain gradient elution.
Explain the autoradiography using radiotracers with a suitable example. What are its advantages and limitations?
What are the essential requirements of a detector in GC
Explain the basic features of ion exchange mechanism. What is the difference between adsorption and ion exchange?
Define resin selectivity. What are the various factors on which selectivity depends?
What are gels Describe the various properties of gels which make them useful for chromatography.
What are polyacrylamide gels? In what way are these different from Sephadex gels?
Define the distribution ratio and percent extraction E and show that 100D/ D+1.
What do you understand by capillary electrophoresis? Explain it schematically.
Explain the terms -Liquid-solid and gas-solid adsorption chromatography.
Explain dialysis and electrodialysis with an illustration for each. In what respects do these differ from each other?
8. Write brief explanatory notes on any three of the following:
Modifier
Reverse Phase Chromatography
DNA Gel Electrophoresis
Donnan Effect
Other Question Papers
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Subjects
- Basic Analytical chemistry
- Electro analytical and Other methods
- Separation methods
- Spectroscopic methods