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BIOCHEMISTRY

Roll No.
BOOKLET NO.
11208


I I


Candidate should write his/her Roll No. in the box above. i Total No. of Questions: 150
Time 2 Hours] No. of Printed Pages 40 [Total Marks 300

INSTRUCTIONS FOR CANDIDATES
1.
All questions are compulsory.

2.
All questions carry equal marks.

3.
The question paper contains 150 questions. The examinee should verify that the requisite number of questions are printed in the question paper, otherwise he should ask for another question paper.

4.
The cover page indicates the number of printed pages in the question paper. The examinee should verify that the requisite number of pages are attached in the question paper, otherwise he should ask for another question paper.

5.
Read carefully the instructions given on the answer sheet supplied and indicate your answers accordingly.

6.
Kindly make necessary entries on the answer sheet only at the places indicated and nowhere else.

7.
Examinees should specially pay attention that 2 marks will be awarded for correct answer.

8.
Examinees should do all rough work on the space meant for rough work on the last page of the question paper and nowhere else and also not even on the answer sheet.


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1. Two sugars which differ from one another only in configuration around a
single carbon atom are termed
(Al Epimers (Bl Anomers
(Cl Optical isomers Stereoisomers
2. Invert sugar is
(Al Lactose
(El Sucrose
(Cl Hydrolytic products of sucrose
Fructose
3. A disaccharide linked by a-1-4-glycosidic linkages is
(Al Lactose (El Sucrose
(Cl Cellulose (Dl Maltose
4. Which of the following polysaccharides is not a polymer of glucose
(Al Amylose (El Inulin
(Cl Glycogen (Dl Cellulose
5. Many globular proteins are stable in solution in spite they lack in
(Al Disulphide bonds (El Hydrogen bonds
(Cl Ionic bonds (Dl Non-polar bonds
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6. At neutral pH, a mixture of amino acids in solution would be predominantly: (Al Non-polar molecules (Bl Positive and monovalent (Cl Dipolar ions
Hydrophobic
7. Edman's reaction can be used to (Al Determine the number of tyrosine residues in a protein (Bl Determine the amino acid sequence of a protein (Cl Determine the number of aromatic amino acid residues in a protein
Hydrolyse the peptide bonds in a protein
8. Bence-Jones protein is lAJ An immunoglobulin (Bl A dimer of heavy chains (Cl A dimer of light chains
A dimer of one heavy and one light chains
9. A fatty acid which is not synthesized in the body and has to be supplied in the diet is
Palmitic acid (Bl Linolenic acid
(Cl Lauric acid Palmitoleic acid

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10. All the following statements describing lipids are true, except

They usually associate by covalent interactions


They are structurally components of membranes


They are an intracellular energy source


They are poorly soluble in water


11. High iodine value of a lipid indicates:

Polymerization Unsaturation


Carboxyl groups Hydroxyl groups


12. Intermediate in the denovo synthesis of triacylglycerols include all the following,
except:

Fatty acyl CoA Glycerol-3-phosphate


CDP diacylglycerol Lysophosphatidic acid


13. In RNA molecule

Allow tRNA to be processed


Are unique to eukaryotic mRNA


Occur at the 3'end of tRNA


Allow correct translation of prokaryotic mRNA


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14.
The mean intracellular concentration of ATP in mammalian cell is about:

(Al 1mM (El 2mM
(Cl 0.1 mM 0.2 mM


15.
If the cytosine content of double stranded DNA is 20% of the total bases, the adenine content will be

(Al 10% 20%
(Cl 30% 40%


16.
The melting temperature of DNA (Tml is (Al Directly proportional to A-T content (Bl Directly proportional to G-C content (Cl Not related to base composition at all CD) Directly proportional to the length of DNA

17.
Deficiency of Vitamin A causes (Al Xerophthalmia (Bl Hypoprothrombinemia (Cl Megaloblastic anemia Pernicious anemIa

18.
Sulpha drugs interfere with bacterial synthesis of (Al Vitamin E (El Co-enzyme A (Cl Lipoate Tetrahydrofolate


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19. The deficiency of which one of the following vitamins causes creatinuria?

Vitamin E Vitamin K


Vitamin A Vitamin B1


20. Biotin is involved in which of the following types of reactions

Deamination Hydroxylation


Carboxylation Oxidation


21. A hormone secreted from posterior pituitary is (Ai Vasopressin

Thyrotropic hormone


Prolactin


Adrenocorticotropic hormone


22. Excessive secretion of adrenocorticotropic hormone causes

Myxoedema Cushing's syndrome


Addison's disease Thyrotoxicosis


2:3. Which of the following events IS not triggered by Glucagon m liver

Stimulation of cyclic AMP Production


Activation of Protein Kinase
(Cl Activation of Glycogen synthase



Activation of Phosphorylase kinase


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24. Which of the following acts directly on the nucleus to produce physiologic effects?

Growth hormone Ghrelin


Dopamine Estradiol


25. Parathyroid hormone is synthesized in

Follicular cells of thyroid glands


Para-follicular cells of thyroid glands


Chief cells of parathyroid glands


Oxyphil cells of parathyroid glands


26. Trypsin belongs to the class (AJ Hydrolase Ligase
Lyase Transferase
27. The rate of most enzyme catalysed reactions changes with pH. As the pH increases, this rate

Reaches a minimum, then increases


Reaches a maximum, then decreases


Increases


Decreases


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28. Succinate dehydrogenase is inhibited by malonate is an example of

Competitive inhibition


Non-competitive inhibition


Uncompetitive inhibition


Feedback inhibition


29. Reaction mediated by co-enzyme pyridoxal phosphate

Acyl transfer Aldehyde transfer


Transamination Oxygenation


30. At high substrate concentration the Michaelis-Menten equation

V will be

Vmax [SJ K
m

V V

Vmax V2Vmax


V [SlYma!Km V


31. The enzyme that splits peptide bonds on the carboxyl side of aromatic amino acid residues, is

Pepsin Trypsin


Chymotrypsin Thermolysin


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32. Pyruvate dehydrogenase complex is regulated by

Covalent modification Allosteric regulation


Both and None of these


33. Allosteric enzymes regulate the formation of products by

Feedback inhibition Non-competitive inhibition


Competitive inhibition Repression-derepression


34. Covalent modification of an enzyme usually involves phosphorylation! dephosphorylation of

Hydroxylysine residue Hydroxyproline residue


Proline residue Serine residue


35.
Which of the following is a substrate specific enzyme?

36.
Frequently employed materials for the adsorption chromatography of proteins include:


Hexokinase Thiokinase
Lactase Aminopeptidase
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High capacity supporting gel


Alumina gel and hydroxyapatite


Starch blocks


All of the above


37. A cation exchange resin linked to cellulose backbone is

CM-Cellulose DEAE-Cellulose


Biogel CD) Sephadex


38. The sorting out of molecules according to size and shape may be adapted to protein purification in this technique

Adsorption chromatography


Gel-filtration chromatography


Paper chromatography


Thin layer chromatography


39. One of the following is used as a ligand in affinity chromatography

Cellulose NAD+


Polyacrylamide Alcohol


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40. In HPLC very high pressure is applied because

The separation will be quick


The particles in the column are extremely small


At atmospheric pressure, the substances resolved will decompose


Without pressure the solvent will move in the opposite direction


41. The factors that affect the electrophoretic movement of particles or molecules are

Size of the particles


Electrical charge on the particles


Solubility in the buffer


Size of the particles and electrical charge on them


42. In Isoelectric focussing, the following substance(s) are used to gwe a pH gradient

Veronal


Tris


Polyamino-polycarboxylic acids


Phosphate buffer


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43.
Fractionation of lipoproteins has been done by using

(Al Spectrophotometer (El Flame photometer (Cl Ultracentrifuge Polarimeter

44.
The sedimentation coefficient is defined as (Al The velocity divided by the centrifugal force (Bl The velocity divided by the centrifugal field (Cl The centrifugal force divided by the effective mass of the particle


The centrifugal field divided by the velocity
45.
Natural radioactive elements belong to one of the series containing (Al Uranium (Bl Thorium (Cl Actinium All of these

46.
Which radioactive isotope is used in the treatment of chronic leukemia

47.
The equation applying Beer's and Lambert's laws relates absorbance with: (Al Concentration of solute and length of the solution column


(Al Radio phosphorus (Bl Radio tantalum
(Cl Radio gold Radio cesium
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(Bl Concentration of solute and height of the solution column (Cl Length and heights of solution column
Intensities of incident and transmitted lights
48. The molar absorption coefficient of benzene is 100 M-1cm-1 at 260 nm. What
concentration would give an absorbance of 1.0 in a 1 cm cell at 260 nm (Al 0.001 M (Bl 0.01 M
(Cl 0.1 M 1.0 M
49. How many proton resonances would you expect to see with ethanol m CCl4 (AlOne (Bl Two (Cl Three (Dl Four
50. The half life of 1311 is
(Al 4 days (Bl 8 days
(Cl 44 days 88 days

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51. For electron microscopy the following is used for staining:

Osmic acid Oil red 0


Bromophenol blue Aniline phthallate


52. A radioactive isotope has a half life of 20 days. Mter 40 days, the amount of the left over substance is the following fraction of the initial isotope
1 1

23 11

4 8
53. Which one of the heaviest particulate component of the cell

Cytoplasm Nucleus


Mitochondria Golgi apparatus


54. The cellular organelles called 'Suicidal bags' are

Nucleolus Golgi bodies


Ribosomes Lysosomes


55. The Golgi complex

Synthesizes proteins


Produces ATP


Provides a pathway for transporting chemicals


Forms glycoproteins


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56.
The largest particulate of the cytoplasm is

(Al Lysosomes (Bl Mitochondria (Cl Golgi apparatus Endoplasmic reticulum

57.
The marker enzyme for mitochondrial fraction isolated by centrifugation IS

(Al Glutamate dehydrogenase (Bl Acid phosphatase
(Cl Glucose-6-phosphatase Phospholipase-a


58.
The RBC III mammals are generally rAJ Uninucleated (Bl Binucleated


Multinucleated Non-nucleated
59. Blood which leaves the liver and moves to the heart has a higher than usual
concentration of
(Al Urea (Bl Bile

Oxygen Carbon dioxide
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60. The pH of blood is about 7.4 when the ratio between NaHC03 and H2C03
IS

101 201


251 301


61. Plasma prothrombin time is prolonged by administration of

Dicoumaral Vitamin K


Prothrombin Vitamin C


62. The carbohydrate of the blood group substance is

Glucose Fucose


Arabinose Maltose


63. Which is not a part of normal adult haemoglobin molecule

Vinyl group Pyrrole rmgs


Methylidene bridges Ferric iron


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64. Sickle cell anaemia is the clinical manifestation of homozygous genes for an abnormal Hb molecule. The mutational event responsible for mutation in the is

Deletion Insertion


Point mutation Crossing over


65. After releasing 02 at the tissues, hemoglobin transports

CO2 and protons to the lungs


02 to the lungs


CO2 and protons to the tissue


Nutrients


66. Hypoxic hypoxia could be due to (AI High p02
Low p02

Low pC02


Defective oxygen III atmosphere


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67. What is the other name of uriniferous tubules?
Cytons Nephridia
Nephrons Neurons
68. Urinary excretion of proteins m 24 hours urme m normal individual IS
approximately
(Al 5-25 mg 25-50 mg (Cl 50-100 mg 100-200 mg


69. Paradoxic aciduria may be seen m the following conditions, except

deficiency
(El Prolonged treatment with steroids



Na+ deficiency


Cushing's syndrome


70.
Glucose absorption may be decreased in

71.
Hydrolysis of dietary triacyl glycerol by pancreatic lipase produce


Oedema Nephritis
Rickets Osteomalitis
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Fatty acids and glycerol


2 Monoacyl glycerol and 2 molecules of fatty acids


Fatty acids and glycerol


Glycerol phosphate and fatty acid


72. D-amino acids are absorbed by

Active transport


In presence of a 'carrier' molecule


In presence of sodium ions


Passive diffusion


73. The following protein is the first one involved in muscular contnicti

Myosin Actin


Calcium-binding troponin Tropomyosin


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74. In the process between: of muscular contraction hydrogen bonds are formed
(Al Troponin and tropomyosin
(El Troponin and actin
Tropomyosin and myosin
Actin and myosin
75. Which of the following is not essential to a reflex arc?


Sensory neuron (El Motor neuron


Synapse Cerebrum


76. Chemical substance concerned with transmission of an impulse from one neuron to next neuron or to a muscle is
Epinephrine Acetylcholine
(Cl Acetylethanolamine (Dl Insulin

77.
Respiratory quotient for mixed diet is

78.
The intake of which food-stuffs result ip the greatest specific dynamic action


0.70 0.75
0.80 0.85
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Carbohydrates Fats


Proteins Minerals


79. Milk is notoriously deficient in which of the minerals

Calcium Iron


Phosphorus Potassium


80. Basal metabolic rate IS elevated III
Hyperthyroidism Undernutrition (el Starvation Hypothyroidism
81. Electron transport chain earners are located in the mitochondria IAl In mitochondrial matrix IE! On the inner surface of the outer mitochondrial membrane (CJ On the outer surface of the outer mitochondrial membrane
In the inner mitochondrial membrane
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82. In oxidative phosphorylation, the oxidation of one molecule :ADPH produces how many ATPs

Zero One


Two Three


83. vVhich of the following statements describing cytochrome oxid"se IS true?

It is a single cytochrome
(Bl It transfers electrons from CoQ to cytochrome b



It transfers four electrons and four protons to form H.)O molecule


It is inhibited by copper


84. The oxidation and phosphorylation in intact mitochondria is blocked by

Puromycin Oligomycin


Gentamycin Streptomycin


85. Which of the following oxidation-reduction systems has highest redox potential

Fe3+ cytochrome bfFe2+


Fe3+ cytochrome afFe2+


Fumarate/succinate


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86. Photorespiration takes place in
Chloroplast Peroxisome
Mitochondria All of these

87. Fructose-2, 6-bisphosphate is formed by the action of

Phosphofructokinase 1
(BI Phosphofructokinase 2
IC) Fructose bisphosphate isomerase



Fructose-I, 6-bisphosphatase


88. An allosteric enzyme responsible for controlling the rate of TCA cycle
IS
Malate dehydrogenase Isocitrate dehydrogenase IC) Fumarase Aconitase
89.
Glycogen IS converted to glucose-I-phosphate by

90.
Glycogenin is (Al Intermediate III glycogen breakdown (El Polymer of glycogen molecules (Cl Protein primer for glycogen synthesis (Dl Uncoupler of oxidative phosphorylation

91.
Gluconeogenesis is decreased by (Al Glucagon (El Epinephrine (Cl Glucocorticoids Insulin

92.
Two important byproducts of HMP pathway are (Al NADH and pentose sugars (Bl NADPH and pentose sugars (Cl FAD and pentose sugar


IA) UDPG transferase Branching enzyme
IC) Phosphorylase Phosphatase
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FADH2 and pentose sugars
93.
Starvation causes a decrease in the rate of

94.
of odd-carbon fatty acid chain produces


(Al Lipogenesis (El Gluconeogenesis
(Cl Lipolysis Glycogenolysis
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Succinyl-CoA Malonyl-CoA


Acetyl-CoA Propionyl-CoA


95. The of 7-dehydrocholesterol is cleaved to form cholecalciferol by

Infrared light


Dim light


Ultraviolet irradiation with sunlight


Light of the tube lights


96. Which of the following regulates lipolysis in adipocytes

Activation of fatty acid synthesis mediated by cAMP


Glycerol phosphorylation to prevent futile esterification of fatty acids


Activation of triglyceride lipase as a result of hormone stimulated increases in cAMP levels


Activation of cAMP production by Insulin


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97. The two nitrogen atoms in urea arise from

Ammonia and glutamine


Glutamine and aspartic acid


Glutamine and glutamic acid
CD) Ammonia and aspartic acid



98. Which of the following amino acids produce a vasodilator on decarboxylation?

Histidine Cysteine


Glutamic acid CD) Arginine


99. Lesch-Nyhan Syndrome IS caused by a complete deficiency of

HGPRTase


Xanthine oxidase


Glucose-6-phosphatase
CD) Glutamyl amidotransferase



100. Homocystinuria is an inherited disorder characterized by

Mental retardation Frequent renal stones


Paralysis of limbs Ectopia lentis


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LOI. The four nitrogen atoms of purines are derived from
Glycine, Ammonia and Aspartate
Glycine, Ammonia and Glutamate
Ammonia, Aspartate and Glutamate
Glycine, Aspartate and Glutamate
102. In humans, the principal catabolic product of pyrimidines is
Uric acid Allantoin
Hypoxanthine
103. Synthesis of GMP from IMP requires the following
Ammonia, ATP
Glutamine, ATP
Ammonia, NADP+, GTP
Glutamine, NADP+, GTP
104. Catabolism of thymidylate gives
a-alanine
a-amino isobutyrate isobutyrate

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105. The essential trace element which catalyzes the formation of Hb in the body
IS
Cu Fe
Mg Mn
106. Iron therapy is ineffective in which of the following conditions?
Chronic blood loss
Inadequate Fe-intake in diet
Hypochromic anaemia of pregnancy
Thalassaemia minor
107. Apart from low levels of serum calcium, tetany can result from a decrease
of:
Potassium Bicarbonate
Phosphate Magnesium
108. Zinc is a constituent of the enzyme
Succinate dehydrogenase Carbonic anhydrase
Aldolase Amylase
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109. What is the normal level of in the serum? 113. The antibiotic which inhibits DNA dependent RNA polymerase IS
(Al 137-148 mEqlL (Bl 120-160 mEqlL
(Cl 3.9-5.0 mEqlL 0.3-0.59 mEqlL
110. Molybdenum is a constituent of
(Al Hydroxylases Oxidases
(Cl Transaminases (Dl Transferases
111. Nicotinic acid is detoxicated in the body with:
(Al Glucuronic acid (Bl Glycine
(Cl Glutamine Cysteine
112. In humans, ammonia is detoxified in liver as
(Al Creatinine Uric acid
(Cl Urea Acetic acid
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Actinomycin D Mitomycin C
Streptomycin Puromycin
114. LDH1 and LDH2 are elevated in Myocardial infarction Liver disease
Kidney disease Brain disease
115. Serum acid phosphatase level increases in Metastatic carcinoma of prostate
Myocardial infarction
Wilson's disease
Liver diseases
116. Overflow aminoaciduria with IS one of the abnormalities observed III patient
Obstructive jaundice Hepatoma
Nephrotic syndrome Acute hepatic necrosis
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117. Relationship between glomerular filtration rate and serum creatinine
concentration is
Non-existent Inverse
Direct Indirect
118. When jaundice results from hepatitis, the unconjugated fraction of total serum
bilirubin is usually
At least 50% More than 50%
Less than 50% Near about 100%
119. Von Gierke's disease is associated with a deficiency of
Glucose-6-phosphate dehydrogenase
Glucose-6-phosphatase in liver
Phosphorylase in muscle
Homogentisic oxidase
.20. Which compound is present in synovial fluid
Hyaluronic acid Chondroitin-4-sulphate
Chondroitin-6-sulphate Heparin

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121. An important cause of water intoxication is
(Al Nephrogenic diabetes insipidus
(El Renal failure
(Cl Gastroenteritis
CD). Fanconi syndrome
122. The antibodies raised against a pure protein will show only one sharp spike
on
(Al Solubility curve (El Solvent precipitation
(Cl Molecular sIevmg (Dl Immuno-electrophoresis
123. The portion of the antigen molecule which is recognized by antibody is known
as
(Al Hapten (El Epitope
(Cl Complement Variable regIon
124. Light chains of immunoglobulins are of the following types
(Al Alpha and Kappa (Bl Alpha and Gamma
(Cl Lambda and Delta Kappa and Lambda
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125. Antigens and haptens have the following similarity
They have high molecular weights
They can elicit immune response by themselves
Once an immune response develops, free antigen and free hapten can
be recognized by the antibody
They can elicit an immune response only in association with some other
large molecule
126. Allergic reactions are mediated by
IgA IgG
IgD IgE
27. Antibody diversity arises from
Gene rearrangement (Bl Gene amplification
Alternative splicing All of these
8. MHC class III proteins include
Immunoglobulins
Components of complement system
T-cells receptors
CD4 and CD8 proteins
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129. The disease of humans acquired immunodeficiency syndrome by IS caused
Bacterium Protozoan
130. 131. Virus Plasmid Hybridoma cells are selected by culturing them in amedium containing Hypoxanthine, aminopterin and thymine Hypoxanthine, aminopterin and thymidine Adenine, hypoxanthine and thymine Guanine, aminopterin and thymidine Genetic material of tobacco mosaic virus is


Single stranded DNA Double stranded DNA


Double stranded RNA Single stranded RNA


132. The concept 'one gene-one enzyme' was based on the experiments conducted on

E.coli Penicillium


Neurospora Drosophila


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133. The eukaryotic nuclear chromosomal DNA
(Al IS a linear and unbranched molecule
(El is not associated with a specific membranous organelle
(Cl not replicated semiconservatively
(Dl IS about of the same sIze as each prokaryotic chromosomes
134. Initiation of protein synthesis begins with binding of
(Al 40S ribosomal unit on mRNA
(El 60S ribosomal unit
(Cl Charging of tRNA with specific ammo acid
Attachment of aminoacyl tRNA on mRNA
35. Sigma and Rho factors are required for
(Al Replication (BJ Transcription
(Cl Translation Polymerisation
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136. During replication, the template DNA is unwound
At one of the ends At both the ends
At multiple sites Nowhere
137. Genetic code was unravelled by
Ochoa-Niranberg Crick-Watson
Beadle-Tatum Sanger-Perutz
138. In biosynthesis of proteins, the chain terminating codons are
UGG, UGU and AUG UAA, UAG and UGA
AAU, AAG and GUA UAA, AUG and UAG
139. Repressor binds to DNA sequence and regulate the transcription. This sequence
is called:
Attenuator Terminator
Anti-terminator Operator
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140. Restriction endonucleases
Cut RNA chains at specific locations
Excise introns from mRNA
Act as defensive enzymes to protect the host bacterial DNA from DNA
of foreign organisms
Remove Okazaki fragments
141. Reverse transcriptase activity is present in the eukaryotic
DNA polymerase ex Helicase
Telomerase DNA polymerase II
142. Binding of RNA polymerase holoenzyme to the promoter region of lac operon
is facilitated by
Catabolite gene activator protein
CAMP
CAP-CAMP complex
None of the above
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143. In Sanger's method ofDNA sequence determination, DNA synthesis is stopped by usmg

2'-dideoxyribonucleoside triphosphates


3'-Dideoxyribonucleoside triphosphates


4'-Dideoxyribonucleoside triphosphates


5'-Dideoxyribonucleoside triphosphates


144. Lambda phage can be used to clone DNA fragments of the sIze

upto 3 kilobases upto 20 kilobases


upto 45 kilobases upto 1,000 kilobases


145. Transgenic animals may be prepared by introducing a foreign gene into

Somatic cells of young animals


Testes and ovaries of animals


A viral vector and infecting the animals with the viral vector


Fertilised egg and implanting the egg into a foster mother


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146. Twenty cycles of PCR can amplify DNA:
220 fold 202 fold
20 x 2 fold 20 fold
147. A particular RNA in a mixture can be identified by

cAl Western blotting
IC) Northern blotting
148. Immobilized enzymes mean
Potentiation of activity
lei Preparation of activity
149. .-grobacterium is very useful in
1.- I Plant genetic engineering
1 C 1 Alcohol fermentation
150. Industrial production of acetic acid uses

Eastern blotting Southern blotting
Presentation of activity All of these
Nitrogen fixation Antibiotic production
(AI Green algae Cyanobacteria
Aerobic bacteria Anaerobic bacteria
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