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BIOTECHNOLOGY



Roll No.
BOOKLET NO.




Candidate should write his/her Roll No, in the box above. i Total No. of Questions: 150
Time: 2 Hours] No. of Printed Pages 32 [Total Marks 300
INSTRUCTIONS FOR CANDIDATES
1.
All questions are compulsory.

2.
All questions carry equal marks.

3.
The question paper contains 150 questions. The examinee should verify that the requisite number of questions are printed in the question paper, otherwise he should ask for another question paper.

4.
The cover page indicates the number of printed pages in the question paper. The examinee should verify that the requisite number of pages are attached in the question paper, otherwise he should ask for another question paper.

5.
Read carefully the instructions given on the answer sheet supplied and indicate your answers accordingly.

6.
Kindly make necessary entries on the answer sheet only at the places indicated and nowhere else.

7.
Examinees should specially pay attention that 2 marks will be awarded for correct answer.

8.
Examinees should do all rough work on the space meant for rough work on the last page of the question paper and nowhere else, not even on the answer sheet.


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1. Cell theory was gIVen by
Kleinfelter Schleiden and Schwann
Waldayer Singer and Nicholson
2. Mitochondria are missing in
Plant cells Animal cells
Bacteria All of these
3. Nucleoid is found in
Algal cells Bacterial cells
Fungal cells Fern cells
4. Plasma membrane IS
Proteinaceous and
(Bl Lipoproteinaceous and non-living
Cellulosic and living
Lipoproteinaceous and living
5. NOR is responsible for
Nucleic acid synthesis CBl Nucleolar organization
Nuclear rearrangement Chromatin thread forinati
6. RNA synthesis is done by

CA) Chromatin thread CB) Karyolymph
Nucleolus CD) Microbodies
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7. Site for protein synthesis IS

Lysosome Smooth endoplasmic reticulum


Ribosomes Mitochondria


8. Active transport involves

ATP RNA


DNA None of these


9. Phragmoplast type of cell di·ision is found in 1.1 Bacteria Animal cells
Plant cells Cyanobacteria
10. Interferons are the defence molecules against

Diabetes


Viral disease


11. Formation of polypeptide chain IS
Transcription
I CI Translation

Blood pressure
Renal failure
Reverse transcription
RNA interference

12. During transcription, messenger RNA contains: (AI Introns Interferons
Exons None of these
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13. Amplification of gene can be done by
(Al Gel electrophoresis (Bl Chromatography
(Cl Rt-PCR HPLC method
14. AIDS is
(Al Bacterial contamination (Bl Virally transmitted
(Cl Inheritable None of these
15. Phosphodiester bonds are found in
(Al Nucleosides (Bl Disaccharides
(Cl Mercaptans !ucleotides
16'. Feulgen reaction is used to test
(Al Proteins (Bl Lipids
(Cl Carbohydrates (Dl Nucleic acid
17. Chi-square test is used for
(Al Standard deviation (El Goodness of fit
(Cl Correlation None of these
18. Replication of DNA takes place during
(Al Prophase (El Telophase
(Cl Interphase Metaphase
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19. DNA ligase helps in the

Process of translation Joining of DNA strands


Joining of Okazaki fragments Synthesis of ATP


20. Operon model was proposed to explain

Eukaryotic transcription Prokaryotic transcription


DNA replication All of these


21. Gene-battery model for eukaryotes was given by
Taylor Britten and Davidson (Cl Harshey and Chase Kleinfelter
22. Which is a marker gene

GUG Luciferase gene


GUS gene Both and


23. Reverse transcription in RNA viruses was given by
Griffith Temin (Cl Wilkins CD) H. G. Khorana
24. Initiation codon is (Al GUG and CAT AUG and GUG
ATC and AUG CD) GAG and ATG
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25. Clover leaf model was gIven for
rn-RNA Operon model
I-RNA r-RNA
2G. Fiest ammo acid to initiate translation IS
Biotin Leucin
Methionine None of these
J . Functional component of gene IS called
IAI Recon Muton
IC) Intron IDI Cistron
28. The best tool for studying molecular diversity is
EFLP RAPD
(Cl SNP All of these
29. PUC stands for
Prevention of uridin
Plasmid developed at t:niversity of California
An oil bug
Pneumonia vIrus
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30.
Taq DNA polymerase is (Al Fragile (Bl Thermosensitive (Cl Thermostable Chemofragile

31.
By submarine gel electrophoresis, DNA fragments are isolated by virtue of: (Al Molecular weight (Bl Equivalent weight

IIIolecular size Source of gene isolation

32.
EDTA normally dissolves at a pH of (Al 7.4 (Bl 8.0 (Cl 8.4 (Dl 7.0

33.
Which of the following statements regarding to a double helical DNA molecule is true? (Al All hydroxyl groups of pentoses are involved in linkages (El Bases are perpendicular to the aXIs (Cl Each strand is identical CDl Each strand replicates itself


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34. Polymorphism is best defined as

Co-segregation of alleles


One phenotype, multiple genotypes


Non-random allele association


One locus, multiple normal alleles


35. After IN damage of DNA in skin

A specific excinuclease detects damaged areas


Purine dimers are formed


Both strands are cleaved


Endonuclease remo'ed the strand


36. Which of the following descriptions of DNA replication is not common to the synthesis of both leading and lagging strands

RNA primer is synthesized


DNA polymerase III synthesizes DNA


Helicase (rep protein) continuously unwinds duplex DNA at the replication fork during synthesis


DNA ligase repeatedly joins the ends of DNA along the growmg strand


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37. Which of the following statements describing restriction endonucleases IS
true?

They always yield overhanging single stranded ends


They recognize methylated DNA sequences


They cleave both strands in duplex DNA


They always yield blunt ends


38. Which of the following enzymes can polymerize deoxyribonucleotides into DNA?

Primase DNA ligase


RNA polymerase III Reverse transcriptase


39. The Singer model of plasma membrane differs from the Robertson's model in the: (AI Xumber of lipid layers

Arrangement of lipid layers


Arrangement of proteins


Absence of proteins in Singer model


40. Which of the following molecules are found III a nucleoside

A pyrophosphate group


A base linked to a pentose sugar


A phosphate group linked to pentose sugar


A phosphate group linked to pentose sugar


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41. Which of the following enzymes can be described as a DNA dependent RNA polymerase

DNA ligase Primase


DNA polymerase III Reverse transcriptase


42. Which one is not a disaccharide sugar

Maltose Sucrose


Fructose Lactose


43. A promoter site on DNA

Transcribes repressor Initiates transcription


Codes for R:A polymerase Regulates termination


44. The consensus sequence 5'TATAAAA3' found in eukaryotic genes is quite similar to a consensus sequence observed in prokaryotes. It is important as the:

Only site of binding of RNA polymerase III


Promoter for all RNA polymerases


Termination site for RNA polymerase II


First site of binding of a transcription factor for RNA polymerase II


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45.
vVhich one of the following binds to specific nucleotide sequences that are upstream of the start site of transcription (Al RNA polymerase Primase (Cl Helicase Histone protein

46.
New proteins destined for secretion are synthesized in the (Al Golgi apparatus (Bl Smooth endoplasmic reticulum (Cl Free polysomes Rough endoplasmic reticulum

47.
How many high energy phosphate bond equindents are utilized in the process of amino acid activation for protein synthesis (Al. Zero (BJ One (Cl Two Three

48.
An inhibitor of protein synthesis that acts as an analogue of amino acyl tRNA is


(Al Mitomycin-C (Bl Streptomycin (Cl Nalidixic acid (Dl Puromycin
49. Uptake by a recipient cell of soluble DNA released from a donor cell is defined as (Al Conjugation Recombination
(Cl Competence Transformation
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50. Direct transfer of a plasmid between two bacteria is defined as

Conjugation Recombination


Competence Transformation


51. Bacterial lipopolysaccharide is characterised by

Phospholipid Ribitol teichoic acid


Glycolipids (waxes) Ketodeoxyoctonate


52. One of the most remarkable aspects of human immune system is its diversity
i.e. the ability to recognize wide range of antigens and to mount a specific antibody response. This is called clonal selection. At cellular level, which is responsible for such specificity?

Cytotoxic T cells


Lymphocytes


Major histocompatibility complex


Specific T cell receptors


53. Which best describes acquired immunity

Increase in C-reactive protein


Presence of natural killer cells


Complement cascade


Maternal transfer of antibody


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54. Which of the following statements best describes haptens

They activate T cells


Penicillin is a hapten


Haptens do not react with specific antibody


Haptens bind the major histocompatibility complex


55. Major role of T cells in the immune response includes

Recognition of epitopes on all surfaces


Complement fixation


Phagocytosis


Production of antibodies


56. Which immunoglobulin is initially seen on the primary response? It is present as monomer on B cell surface but as a pentamer in serum
IgG IgM
IgE IgA
57. Which immunoglobulin IS pnmary antibody in saliva, tears and intestinal and genital secretions
IgG IgM
IgE IgA


58. A xenograft is best described as (Al Transplant from one regIOn of a person to another

Transplant from one person to genetically identical person (Cl Transplant from one specIes to the same specIes


Transplant from one specIes to another species


59.
An idiotype is characterized by (Al Determinant exposed after papam cleavage to an F(ab'l2 fragment (El Determinant from one clone of cells and probably located .close to the

antigen binding site of immunoglobulin (Cl Determinant inherited in I1Iendelian manner and recognized by cross immunization of individuals in a species CDl Heavy chain determinant recognized by heterologous antisera

60.
Penicillin IS commercially produced from


(Al Penicillium chrysogenum (El Penicillium notatum (Cl Penicillium griesus Penicillium wentii
61. The proteases used in detergent industry are
(Al Acid proteases eEl Neutral proteases
(Cl Alkaline proteases (Dl None of these
........... ....,.,... " ........ ,........

62.
An enzyme that catalyzes the reaction A B changes the (Al Heat of reaction (Bl Equilibrium constant (Cl Rate of forward and reverse reactions (Dl Entropy of the reaction

63.
Diacylglycerol activates which of the following enzymes

(Al Protein kinase A (Bl Protein kinase C (Cl ;1.AP kinase lDl Tyrosine kinase

64.
Cellular proteins destined for secretion are packaged and sorted in (Al Lysosomes (Bl Endosomes (Cl Transgolgi network (Dl Endoplasmic reticulum

65.
Which process leads to the formation of polytene chromosomes? (Al Repeated replication without separation of chromatids (Bl Recombination between adjacent chromosome segments (Cl Sister chromatid exchange (Dl Non-disjunction of chromatids during meiosis

66.
In animals, the enzyme unique to gluconeogenesis is (Al Enolase (Bl Phosphoglyceromutase (Cl Fructose bisphosphatase (Dl Aldolase


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67. Which of the following does not make direct use of pH or proton gradient?
Mitochondria Chloroplast
Bacterial flagellum Protozoan flagellum
68. Deletion or addition of a nitrogenous base in the reading frame leads
into
Switches Inversion
Frame shift Translocation
69. Nucleosome is an
Oligonucleotide
Octamer of non-histones
Euchromatic region with poor heterochromatin
Octamer of histones complexed with DNA
70. Stationary phase in TLC IS
Whatman Paper I Silica gel
Lipid layer Phenol and ether
71. X-ray crystallography was used to determine
3D structure of plasma membrane
Double helix of DNA
Structure of pigments
None of the above
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72. Barbara McClintock is credited for

Solanoid model


• Iron alum acetocarmine method


Transformation


Jumping genes


73. Isolated DNA can be studied quantitatively by

Gel electrophoresis UV-VIS spectrophotometry


Column chromatography CD) PCR


74. Which is the most effective mutagen?

Ionizing radiations Colchicine


Infrared rays Maleic hydrazide


75. Which is a prokaryote?

Vibrio Nostoc


Pseudomonas All of these


76. Most resistant structure in bacterium is

Proteinaceous cell-wall Nucleoid


Endospore Plasmid


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77.
Agarophytes are the members of (Al Freshwater algae (Bl Slime molds (Cl Marine water algae Ferns

78.
Which of the following is a biocontrol agent (Al Cephaleuros (Bl Trichoderma (Cl Rhizobium Nematode?

79.
Bacterial transformation was demonstrated by Griffith using: (Al Lectobacillus (Bl Streptococcus (Cl Diplococcus Vibrio

80.
An undifferentiated mass of cell is called (Al Axillary bud (Bl Embryoids (Cl Callus Implant

81.
Cryopreservati'on is commonly done by using (Al Chilled ethanol (Bl CNG (Cl Liquid LPG

z

82.
Laminar air flow receives air through (Al Microfilter (Bl Silica filters (Cl HEPA filters Synthetic filters


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83.
Best example of somatic clones is (Al Mulberry (Bl Arabidopsis (Cl Sugarcane Basmati rice

84.
Gene gun IS used to (Al Replicate genes (Bl Mutate genes (Cl Transfer genes Isolate genes

85.
Tumour in roots can be de"eloped by (Al Nitrosomonas (El Ti-plasmids (Cl RNAi Clostridium

86.
Semen bank can be developed by preservmg semen m (Al Formalin (Bl Formalin Acetic Alcohol (FAAl (Cl 70% Alcohol Liquid Nitrogen

87.
Fermentation is (Al Aerobic process with low energy output (Bl Aerobic process with high energy output (Cl Anaerobic process with low energy output (Dl Anaerobic process with high energy output


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88. Ozone depletion can be prevented by (Al Lowering the emISSIOn of CFC gases (Bl Preventing deforestation (Cl Reducing pollutants
All of the above
89.
Enzymes are basically (Al Metals IE I Polysaccharides (Cl Proteins I Thermoresistant

90.
Optimum temperature for enzyme acti'ity is

(Al 00_5°C
(Cl 35°-60°C 600 and abo'e


91.
Nitrocellulose membrane is used for (Al Electrophoresis (Bl Chromatography (Cl Blotting (Dl Spectrophotometry

92.
For measuring length of DNA fragments upto 1000 bp. the suitable marker IS (Al 100bp ladder (Bl Hind III i. (Cl Both (Al and (Bl None of these


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93.
Best ecofriendly feedstock for biofuel production is

(Al Jatropha Zea mays
(Cl Algae Sugarcane


94.
Byproduct of sugarcane industries can be used for the production of (Al Pharmaceuticals (Bl Menthol (Cl Ethanol None of these

95.
Transgenic cropo are being criticized because of


Biodi"ersity loss
(B1 Ethical reasons
(Cl Suspected harm to human race



All of the above



96.
Photobioreactors are meant to (Al Grow algae (Bl Utilize COemission for photosynthesis


2
(Cl Production of green energy feedstock
(Dl All of the above

97. IPR issues are essential for
(Al Biosafety (Bl Environmental conservation
(Cl Both of the above None of these
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98. Facultative saprophytes are basically
Saprophyte Parasite
Symbionts None of these
99. Plant viruses usually contain
dsDNA dsRNA
ssDNA ssRNA
100. Chimeric DNA is
A plasmid having multiple foreign DNA segments
A cosmid with single foreign DNA segment
Zea mays with variously coloured grains
None of the above
101. Peptidoglycan and Teichoic acid are the components of cell wall ill
. Cyanobacteria Trichoderma
Pteridium Bacteria
102. Pars amorpha, pars granulosa and pars nucleonema are the major parts
of:
Golgi bodies Plasmalemma
Chromatin Nucleoli
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103. Terminal oxidation occurs m
Grana lamella Stroma
F1 particles Quantasomes
104. During photosynthesis, oxygen is released by the process of
Cyclic photophosphorylation Electron transport
Photolysis of water Reduction of NADP
105. Rate of photosynthesis is negligible
Red light IBI Blue light
Green light Polychromatic light
106. Svedberg unit is associated with
Lysosome Chromomeres
Ribosomes Nucleosome
107. Attachment of ribosomes to the membrane of endoplasmic reticulum IS
associated to
ATP and NAD concentration
(Cl concentration concentration
108. Under anaerobic conditions, net output of ATP in glycolysis is

36 10



(El 2
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109. Transposon is the segment of DNA responsible for
Homologous recombination
Non-homologous recombination
Amitosis
Chromosomal non-disfunction
110. Centipede model of chromosome was proposed by
Ris Taylor
Roger and Kornberg Godward
111. Mitochondria can be stained with
Acetocarmine lEI Geimsa
Tanus green B Basic fuchsin
112. Cell sap is the fluid present in
Mitochondria Chloroplast
Endoplasmic reticulum Cell vacuole
113. Buffer solutions are
Usually acidic
Usually basic
Able to resist change in pH
Unable to resist change in pH
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114. Preventive vaccine by rDNA technique has been developed for
Malaria Typhoid
Hepatitis Tuberculosis
115. ATP is a type of
Polysaccharide Lipoprotein
Nucleotide Electron acceptor
116. SDS-PAGE is meant for the separation of
1.- IBI
Proteins Lipids
117. Camera lucida is used for
Gel photography Microphotography
Phase contrast photography Microscopic object drawings
118. Terminalization is the characteristic feature of
Pachytene Diakinesis
IC) Leptotene Zygotene
119. Tris base has the molecular formula
CIHP)n CjHllNOS
C22H40);4Mg NaHzP04
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120. Isolated DNA is considered as pure if
A2601A280 is more than 1.7
A260lA280 is between 1.7 to 1.9
A260lA280 is less than 1.7
This ratio has nothing to do with purity
121. Which of the following is called DNA scissor?
RNA polymerase DNA polymerase
Restriction endonuclease DNA ligase
122. Gram +ve bacteria have in the cell wall
Less lipid and high peptidoglycan
More lipid and less peptidoglycan
Equal amount of lipids and peptidoglycan
None of the above
123. Cobalt is a
Macronutrient Micronutrient
Energy source Gelling agent
124. Biochemical test of proteins can be done by
Xanthoproteic test Biuret test
Xanthine oxidase test Both and

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125. Substances having the capacity to react with immune system and mount an
immune response along with allergic manifestation are
Immunogens Antigens
Antibodies None of these
126. Which is called as Drosophila of plant world?
Saccharomyces Acetabularia
Neurospora E. coli
127. In the alphabet stands for

Induction Inversion
Interference Incubation
128. Which point mutation IS less harmful
Deletion Insertion
Substitution All of these
129. Which is not a pathogen
rA) Mycoplasma Lactobacillus
ie, Salmonella Pneumococcus
130. Fission fungi is the name gIven to
1.- Saccharomyces Cyanobacteria
Saprolegnia Bacteria
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131. Double stranded, circular DNA, not protected by nuclear membrane and wIthout
nucleolus is called
(Al Nucleoid (Bl Genophore
(Cl Incipient nucleus All of these
132. Leghaemoglobin is
(Al Blood of non-chordates
(Bl A type of blood cell
(Cl Iron containing pigment III nitrogen fixing root nodules
A component of cytochrome
133. Pure plant cultures can be obtained by
(Al Somatic embryogenesis Haploid cultures
(Cl Protoplast fusion Cybridization
134. Axenic cultures are
(Al In-uitro cultures (Bl In-uiuo cultures
(Cl Contamination free cultures Micropropagation
135. Eutrophication is related to
(Al Freshwater bodies
(Bl Water bodies with high orgamc matter
(Cl A dense forest ecosystem
A process of eugenics
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136. Fertile layer of the soil IS
Subsoil Top soil
Both and None of these
137. Radioactive pollution hazard was most severely suspected recently
In
South America The Netherlands
Japan Armenia
138. Soilless cultures are knmvn as
Micropropagules Mangroves
Hydroponics Implants
139. Carbon sequestration can be done by
Euglena
Sulphur bacteria
Photosynthetic microorganisms
Mycoplasma
140. Vinegar is obtained through
Broth culture Aerobic respiration
Fermentation Bioremediation
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141. During Calvin cycle, COacceptor is
2
(Al Phosphoglyceraldehyde (Bl Ribulose 5 diphosphate (Cl u-ketogluteric acid Pyruvic acid
142. Krenz anatomy is found in
(Al C3 plants (Bl C4 plants
(Cl All microorganisms (Dl Dicoteledons only
143. Secondary metabolites
(Al Constitute the plant body
(Bl Synthesized and stored in plant parts
(Cl Are of secondary use and of no commercial value
End products of metabolic reactions
144. Most popular medicinal plant Aloe vera IS a
(Al Mesophyte (Bl Hydrophyte
(Cl Xerophyte Mangrove plant
145. Antioxidants:
(Al Add toxic oxygen to the body
(Bl Inactivate reactive oxygen
(Cl Reduce cellular respiration
Prevent dehydration

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146. Language of gene is called as
(Al Nucleoside (Bl Gene sequence
(C1 Genetic code (Dl Operon
147. Which IS naturally acquired active immunity?
(Al Measles or Chicken pox (Bl Filaria
(Cl Hepatitis IgG
148. Which of the following is commercially produced through fermentation
(Al Vitamin C (Bl Vitamin D
(Cl Vitamin BIZ Penicillin
149. A eutrophicated pond has the BOD
(Al Optimum (Bl Very high
(Cl Very low None of these
150. Gobar gas is produced from dung and is basically

Carbon mono-oxide (Bl Hydrogen sulphide
c :Iethane Butane
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