M.Sc. DEGREE EXAMINATION, APRIL 2016
Second Semester
Biotechnology
PLANT MOLECULAR BIOLOGY
(CBCS 2013 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 2 20)
Answer all questions.
1. Genome.
2. Gene.
3. STS.
4. Cryopreservation.
5. T-DNA.
6. Ti-plasmid based vector.
7. Salinity.
8. PHB.
9. Reporter genes.
10. Herbicide.
Sub. Code
501203
RW-10860
2
Wk 10
Part B 5 25)
Answer all questions choosing either or
11. Write short notes on architecture of plant genome.
Or
Give an account on promiscuous DNA.
12. Write short notes on RAPD technique.
Or
Explain cryopreservation method.
13. Write short notes on agrobacterium.
Or
Give an account on super binary Ti-plasmid vectors
for plant transformation.
14. Write short notes on the direct methods of gene
transfer in to plant cells.
Or
What are the advantages and disadvantages of
genetically modified crops?
15. What are the selectable markers used in plant gene
expression vectors? Explain.
Or
Give an account on phytoremediation.
RW-10860
3
Wk 10
Part C 10 30)
Answer any three questions.
16. Illustrate the structural features of a representative
plant gene with functions.
17. Explain in detail about the following molecular markers
for plant identification improvement arid analyzing
genetic diversity
RAPD
Microsatellites.
18. Give a detailed account on agrobacterium mediated
transformation of food crops.
19. Write notes on
transposon tagging
molecular farming and case studies.
20. How will you produce viral resistant plants using genetic
engineering?