M.Sc. DEGREE EXAMINATION, APRIL 2016
Biotechnology
Lab II MICROBIOLOGY
(CBCS 2013 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 2 20)
Answer all questions.
All questions carry equal marks.
1. What are the safety measures in microbiology
laboratories?
2. Define TEM.
3. Define simple staining
4. Write a short note on hanging drop method
5. Gram positive bacteria
6. Describe about growth rate and generation time
7. Explain bioreactor
8. Define probiotics
9. Define IMVIC tests
10. Oxidase test
Sub. Code
501106
RW-11052
2
Ws1
Part B 5 25)
Answer all questions choosing either or
11. Describe the principle and application of SEM.
Or
Explain in detail about micrometry method.
12. How will you determine the motility of bacteria?
Or
Write notes on negative staining
13. Give an account on the enzyme producing
microorganisms with example.
Or
Describe the factors that affecting bacterial growth.
14. Explain bacterial cell-cell communication system.
Or
Discuss about MPN method.
15. How will you enumerate bacteria and fungi from
soil sample?
Or
Demonstrate catalase and oxidase test.
RW-11052
3
Ws1
Part C 10 30)
Answer any three questions.
All questions carry equal marks.
16. Illustrate the principle and applications of phase contrast
and fluorescence microscopy.
17. Discuss about the techniques used for isolation of pure
culture.
18. Explain the screening and identification of enzymes
DNase
Gelatinase
Lipase
Protease
Amylase
19. Write an essay on antibiotic assay and antibiotic
resistance — in detail.
20. Describe the molecular methods in identification of
culture dependent and independent bacterial organisms.M.Sc. DEGREE EXAMINATION, APRIL 2016
Biotechnology
Lab II MICROBIOLOGY
(CBCS 2013 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 2 20)
Answer all questions.
All questions carry equal marks.
1. What are the safety measures in microbiology
laboratories?
2. Define TEM.
3. Define simple staining
4. Write a short note on hanging drop method
5. Gram positive bacteria
6. Describe about growth rate and generation time
7. Explain bioreactor
8. Define probiotics
9. Define IMVIC tests
10. Oxidase test
Sub. Code
501106
RW-11052
2
Ws1
Part B 5 25)
Answer all questions choosing either or
11. Describe the principle and application of SEM.
Or
Explain in detail about micrometry method.
12. How will you determine the motility of bacteria?
Or
Write notes on negative staining
13. Give an account on the enzyme producing
microorganisms with example.
Or
Describe the factors that affecting bacterial growth.
14. Explain bacterial cell-cell communication system.
Or
Discuss about MPN method.
15. How will you enumerate bacteria and fungi from
soil sample?
Or
Demonstrate catalase and oxidase test.
RW-11052
3
Ws1
Part C 10 30)
Answer any three questions.
All questions carry equal marks.
16. Illustrate the principle and applications of phase contrast
and fluorescence microscopy.
17. Discuss about the techniques used for isolation of pure
culture.
18. Explain the screening and identification of enzymes
DNase
Gelatinase
Lipase
Protease
Amylase
19. Write an essay on antibiotic assay and antibiotic
resistance — in detail.
20. Describe the molecular methods in identification of
culture dependent and independent bacterial organisms.