DISTANCE EDUCATION
M.Sc. (Botany Specialization Plant Bio technology)
DEGREE EXAMINATION, MAY 2016.
PLANT GENETIC ENGINEERING
(2008 onwards)
Time Three hours Maximum 100 marks
Answer any FIVE questions.
Each question carries 20 marks.
20
1. Discuss about gene cloning strategies.
Briefly describe the recombinant DNA technology.(8)
Discuss the brief history of recombinant DNA
technology.
2. Give an account on selectable marker genes.
Explain 30S promoter of CaMV.
Discuss the role of reporter genes in optimizing
transformation.
3. Write detailed about Agrobacterium mediated gene
transfer.
Write short notes on golden rice.
Give an account on electroporation.
4. Discuss about herbicide resistance genes.
Explain the gene involved in delayed fruit
ripening.
Cytoplasmic male sterility- Discuss.
Sub. Code
25
DE-3858
2
wk10
5. Explain SCAR.
Discuss about chloroplast engineering.
Write short notes on transplastontic plants.
6. Write an account on chemical gene transfer
methods.
Explain biolistics.
Write short notes on microsatellites.
7. Write short notes on Antisense RNA.
Discuss about plant genomic projects.
Write short notes on cloning vectors.
8. Discuss about cross protection satellite RNA.
Write detailed about antibiotic sensitivity assay.
Write short notes on alkaline phosphatase.