[LO 0219] FEBRUARY 2019 Sub. Code: 5016
B.Sc. MEDICAL LABORATORY TECHNOLOGY
SECOND YEAR
PAPER I HISTOPATHOLOGY CYTOLOGY TECHNIQUES
Q.P. Code: 725016
Time: Three Hours Maximum: 100 Marks
Answer all questions
I. Elaborate on: x 10 30)
1. Describe in detail about Automation in Histopathology laboratory.
2. Enlist the stains used in Cytology. Describe in detail the composition, preparation,
staining procedure and advantages of May Grunwald Giemsa staining.
3. List the various types of microtomes. Discuss in detail the procedure of section
cutting. Add a note on the problems faced during section cutting and their
troubleshooting.
II. Write notes on: x 5 40)
1. Periodic Acid Schiff stain.
2. Factors affecting fixation of tissues.
3. Cell block preparation and its applications.
4. Classify decalcifying agents.
5. Uses and care of Cryostat.
6. Types of Embedding media.
7. Eosin and its substitutes.
8. Museum mounting techniques.
III. Short answers on: (10 x 3 30)
1. Uses of FNAC.
2. Two adhesives used in histopathology.
3. Glutaraldehyde fixative.
4. Mordants.
5. Floaters.
6. Cells seen in Vaginal smear.
7. Water bath-Temperature and use in histopathology.
8. Differentiation in tissue processing.
9. Surface decalcification.
10. Uses of Masson Fontana staining.