THE TAMIL NADU DR. M.G.R. MEDICAL UNIVERSITY
(LO 2035) MARCH 2019 Sub. Code: 2035
B.PHARM. DEGREE EXAMINATION
PCI Regulation SEMESTER III
PAPER III PHARMACEUTICAL MICROBIOLOGY
Q.P. Code: 562035
Time: Three hours Maximum: 75 Marks
I. Elaborate on: Answer any TWO questions. x 10 20)
1. Discuss various preservation methods of Pure culture.
2. Give a detailed note on cultivation of Viruses in laboratory.
3. Explain the types of Laminar air flow equipment and its operating principles with
suitable diagram.
II. Write notes on: Answer any SEVEN questions. x 5 35)
1. Explain briefly about the structures internal to bacterial cell wall with diagram.
2. Write the principle and procedure of Gram staining method.
3. Indole production test.
4. Write a note on Radiation sterilization.
5. Classification of Fungi.
6. Explain briefly about the various factors influencing disinfectant action.
7. What is Minimum Inhibitory Concentration? Explain the assay of Antibiotics by
Turbidimetric method.
8. Write a note on various sources and types of Microbial contaminants.
9. Application of Primary cell culture.
III. Short answers on: Answer ALL questions. (10 x 2 20)
1. Tyndallization.
2. Classification of bacteria based on temperature.
3. Use of culture media.
4. Principle of Acid fast staining method.
5. Dry heat sterilization.
6. What is Chick-Martin test?
7. Distinguish Antiseptics and Disinfectants.
8. Give the principle of Microbial assay.
9. Any two factors affecting Microbial spoilage.
10. Transformed cell culture.