Seat No.: Enrolment
GUJARAT TECHNOLOGICAL UNIVERSITY
M.PHARM SEMESTER EXAMINATION -WINTER 2018
Subject Code: MPG103T Date: 05/01/2019
Subject Name: Phytochemistry
Time: 10:30 AM TO 01:30 PM Total Marks: 80
Instructions:
1. Attempt any five questions.
2. Make Suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1

Define Tracer Techniques. Discuss various methods used in Tracer Techniques.
06

Discuss advantage, limitation requirement of Tracer Techniques.
05

Describe various applications of tracer techniques.
05
Q.2

Explain schematic diagram for isolation characterization of vinca alkaloids.
06

Describe characterization and purification of sennosides from senna leaves.
05

Explain characterization and purification quercitin phytoconstituent.
05
Q.3

Discuss history of herbs as source of drugs and drug discovery.
06

Explain how separation of phytoconstituents done by SCFE techniques.
05

Give emphasis on phases of clinical trials protocol design for lead molecules.
05
Q.4

Explain discovery process, drug registration, selection and optimization
of lead compounds.
06

Discuss selection and optimization of artemesin andrographolides.
05

Explain separation of phytoconstituents by CCCET techniques.
05
Q.5

Describe recent advances in extractions methods and give choice of solvent used for extraction.
06

Discuss advance methods used for fractionation of phytoconstituents.
05

Explain schematic flow chart for extraction purification of Glycyrrhizin.
05
Q. 6

Explain separation of phytoconstituents with help of preparative HPLC flash column chromatography.
06

Enumerate various HPTLC applications which help in the characterization of herbal extracts.
05

Discuss various LCMS/GCMS applications used in the characterization of herbal extracts.
05
Q.7

Give Structure elucidation of menthol by UV& IR.
06

Give Structure elucidation of kaempferol by MS NMR.
05

Explain structure elucidation of caffeine by UV IR.
05