B. Pharmacy IV/ I-Semester (Suppl.) Examination
Subject Pharmaceutical Analysis II (Instrumental Methods of Analysis)
Time 3 Hours Max. Marks: 70
Note: Answer all questions. All questions carry equal marks.
1 Define chromphore and auxochrome and give examples of conjugatged
chromphores and auxochromes.
Give the description and working of UV-visible spectrophotometer with a neat
labeled diagram.
OR
Explain any two methods for single component analysis by spectrophotometry.
State and explain Beer-Lambert's law and its deviations in applications.
Make a note on different types of solvents used for UV-spectrophotometric
analysis.
2 State and explain Hooks law.
Give the description and working of IR-spectrophotometer.
OR
Write about molecular vibrations in IR spectroscopy.
How do you do interpretation of IR spectra of organic compounds? Explain in brief.
3 Write about the following:
Chemical shift
Spin-spin coupling
Solvents used in NHR spectroscopy
OR
Give the theory and principles of mass spectroscopy techniques.
Explain the radiative and non-radiative phenomena with help of Jablonski diagram.
4 Give the description and working of standard Hydrogen Electrode.
Write about different end point evaluation methods in potentiometric titrations.
OR
Write short notes on Turbidometry and Nephelometry.
Explain about different types of conductimetric titrations.
5 Write about the following:
Column chromatography
Paper chromatography
OR
Discuss the theory and principles involved in HPLC separations.
Write about different types of detectors used in gas chromatography.
Give the principles of electrophoresis technique.