FACULTY OF PHARMACY
M. Pharmacy (Pharm. Chemistry) II-Semester (Main) Examination,
August 2018
Subject Advanced Spectral Analysis
Time: 3 Hours Max. Marks: 75
Note: Answer any five questions. All questions carry equal marks.
1 Discuss any two 2DNMR (NOESY, COSY, HETCOR) techniques of and explain
their importance in structural interpretation of organic compounds.
2 Write the wood ward Fieser rules for -Carbonyl Compounds and 3
butadiene's. Calculate max for the following compounds.
Explain the importance of meta stable ion peaks and isotopic peaks in mass
spectroscopy.
3 Discuss the principle, instrumentation and applications of any two of the following
LC-MS HPTLC Super Critical fluid Chromotography.
4 Explain principle instrumentation and applications of any two of the following
techniques
DSC TGA Raman Spectroscopy
5 Discuss the fragmentation of important functional groups in mass spectroscopy.
Explain MC Lafferty rearrangement and ring rule
6 Explain the principle and applications of any two of the following instrumentation
techniques:
Flash chromcotography
LC-FTIR
Ion exclusion chromatography
7 Write a note on any two of the following
ELISA and its applications
Radioiumuno assay of insulin
Bioassays
..2
OU 1701 OU 1701
Code No. 1221/PCI

8. Draw a rough 'HNMR Spectra for the following compounds with proton splitting
How do you interpret the following functional groups in IR? (any six)
NO2 ii) SO2 iii) NH2 iV) R
O IIC
OR CONH
Vi) COOH Vii) CN Viii) CHO ix)
O IIC
OH