BOT/VI/12 Student's Copy
2 0 1 8
6th Semester
BOTANY
TWELFTH PAPER
Plant Biotechnology and Experimental Embryology
Full Marks 55
Time 2½ hours
PART A—OBJECTIVE
Marks 20
The figures in the margin indicate full marks for the questions
SECTION—A
Marks 5
Tick the correct answer in the brackets provided 1×5=5
1. The first restriction endonuclease isolated from an strain of Escherichia
coli is
BamHI
FokI
HindII
EcoRI
/516 1 Contd.
2. Enzyme that allows the synthesis of DNA from an RNA template is called
RNA polymerase
Restriction endonuclease
Reverse transcriptase
DNA polymerase
3. Cryopreservation of tissue with liquid nitrogen is done at which
temperature?
-196 °C
-126 °C
-206 °C
-96 °C
4. Which of the following is known as 'FlavrSavr'?
Specific variety of pesticide
Golden rice
Transgenic tomato
Transgenic cotton
5. Which of the following is the most commonly used method for the culture
of plant protoplasts?
Bead culture
Agar embedded culture
Feeder layer technique
Hanging drop method
BOT/VI/12/516 2 Contd.
SECTION—B
Marks 15
Write notes on the following 3×5=15
1. Methylase
2. Reporter gene in plants
3. Macronutrients of MS medium
4. Plantibodies
5. Cybrid
PART B—DESCRIPTIVE
Marks 35
The figures in the margin indicate full marks for the questions
1. Define PCR. Explain the steps of PCR with diagram.
OR
Write notes on the following 3½+3½=7
Cloning vectors
Ligases
2. Describe the enzymes used in molecular cloning. 7
OR
Write notes on the following 3½+3½=7
Gene gun
Agrobacterium-mediated transformation in plants
BOT/VI/12/516 3 Contd.
3. What are explants? Describe the factors considered for the selection of
explants.
OR
Write notes on the following 3½+3½=7
Cryopreservation
Totipotency
4. Describe the role of biotechnology in agriculture. 7
OR
Write notes on the following 3½+3½=7
Bt cotton
Golden rice
5. What is micropropagation? Mention the advantages and disadvantages of
micropropagation.
OR
Write notes on the following 3½+3½=7
Isolation of protoplasts
Embryo culture