ZOO/VI/12 Student's Copy
2 0 1 8
6th Semester
ZOOLOGY
Paper ZL-XII
Biotechnology and Bioinformatics
Full Marks 55
Time 2½ Hours
PART A—OBJECTIVE
Marks 20
The figures in the margin indicate full marks for the questions
SECTION—A
Marks 5
Tick the correct answer in the brackets provided 1×5=5
1. Each individual has a unique DNA fingerprinting as individuals differ in
number of minisatellites
size of minisatellites
location of minisatellites
All of the above
/511 1 Contd.
2. The first cloning vector with circular DNA molecule discovered from E. coli
was
plasmid
yeast artificial chromosome
cosmid
bacteriophage lambda
3. Which process is used to insert normal genes into human cells to correct
disorders?
Stem cell therapy
Gene therapy
Molecular cloning
cDNA library
4. A software that manages the computer hardware, and provides common
services for execution of various application softwares is
resource management
information retrieval
job management
operating system
5. European Molecular Biology Laboratory (EMBL) mainly deals with
nucleotide sequence
amino acid sequence
genome sequence
protein sequence
ZOO/VI/12 2 Contd.
SECTION—B
Marks 15
Write short notes on the following in 5 to 8 sentences each 3×5=15
1. Western blotting
2. Cloning vectors
3. Applications of genetic engineering in medicine
4. Information retrieval
5. Internet tools
PART B—DESCRIPTIVE
Marks 35
The figures in the margin indicate full marks for the questions
1. There is a technique for selectively amplifying particular segments of DNA.
Write an account of the principle, components and protocol of this
technique with suitable illustration. 1½+1½+4=7
OR
Differentiate between gene sequencing and genomic sequencing. Explain
the process of genome sequencing.
2. What do you mean by genetic engineering? Give a brief account on the
basic procedure with a note on its major applications.
OR
Write an account on the enzymes used in genetic engineering. 7
ZOO/VI/12 3 Contd.
3. What is gene library? Differentiate between genomic library and cDNA
library. Write the basic steps of construction of gene library.
OR
Give a brief account on the applications of genetic engineering in
agriculture. 7
4. Write an account on the concept of bioinformatics. Write a note on basic
operating systems.
OR
Write notes on the following 3½+3½=7
Databases
Internet for biologist
5. What do you mean by genome database? Write an account on NCBI.
OR
What is proteome database? Give an account on BLAST.