BC/III/03 Student's Copy
2 0 1 8
Pre-CBCS
3rd Semester
BIOCHEMISTRY
THIRD PAPER
Enzymology and Metabolism—II
Full Marks 55
Time 2½ hours
PART A—OBJECTIVE
Marks 20
The figures in the margin indicate full marks for the questions
SECTION—A
Marks 5
Tick the correct answer in the brackets provided 1×5=5
1. Enzymes work by
reducing the activation energy
increasing the total energy of substrate
increasing the equilibrium constant
increasing the total energy of the product
/226 1 Contd.
2. The Km value of an enzyme is
the substrate concentration at half maximum velocity
half the substrate concentration at maximum velocity
dissociation constant of the enzyme-substrate complex
the total enzyme concentration
3. The defective enzyme in alkaptonuria is
tyrosinase
phenylalanine hydroxylase
homogentisate oxidase
cystathione synthase
4. Which component of ETC contains an iron-sulphur centre?
Coenzyme Q
Cytochrome C
NADH dehydrogenase
Succinate dehydrogenase
5. The parent nucleotide in the synthesis of purine nucleotide is
xanthine monophosphate
hypoxanthine
glycinamide ribosyl phosphate
inosine monophosphate
BC/III/03/226 2 Contd.
SECTION—B
Marks 15
Write short notes on the following 3×5=15
1. Activation energy
2. Significance of Km and Vmax
3. Glycogenic and ketogenic amino acids
4. Uncouplers of oxidative phosphorylation
5. Lipid malabsorption
PART B—DESCRIPTIVE
Marks 35
The figures in the margin indicate full marks for the questions
1. Write a detailed note on the transition state theory of enzymes. 7
OR
Write the IUB system of classification of enzymes giving suitable
examples.
2. Derive the Michaelis-Menten equation for uni-substrate reactions. 7
OR
Write notes on the following 3½+3½=7
Competitive enzyme inhibition
Allosteric enzymes
BC/III/03/226 3 Contd.
3. Explain the steps involved in the urea cycle. 7
OR
Write notes on the following 3½+3½=7
Phenylketonuria
Albinism
4. Discuss the components of the electron transport chain of
mitochondrial membrane. 7
OR
Write short notes on the following 3½+3½=7
ATP synthase
P O ratio
5. Discuss the degradative process of purine nucleotides. 7
OR
Write short notes on the following 3½+3½=7
Gout
Hyperglycemia